In vitro activity of the protegrin IB‐367 alone and in combination compared with conventional antifungal agents against dermatophytes. Issue 4 (16th October 2013)

• Record Type: Journal Article
• Authors: Simonetti, Oriana; Silvestri, Carmela; Arzeni, Daniela; Cirioni, Oscar; Kamysz, Wojciech; Conte, Irene; Staffolani, Silvia; Orsetti, Elena; Morciano, Angela; Castelli, Pamela; Scalise, Alessandro; Kamysz, Elzbieta; Offidani, Anna Maria; Giacometti, Andrea; Barchiesi, Francesco
• Is Part Of: Mycoses. Volume 57:Issue 4(2014)
• Page Start: 233
• Page End: 239
• Publication Date: 2013-10-16
• Subjects: Pathogenic fungi -- Periodicals; Medical mycology -- Periodicals; 616.969
• DOI: 10.1111/myc.12148
• Languages: English
• ISSNs: 0933-7407

Abstract

<abstract abstract-type="main" id="myc12148-abs-0001"> <title>Summary</title> <p>The occurrence of resistance or side effects in patients receiving antifungal agents leads to failure in the treatment of mycosis. The aim of this experimental study was to investigate the <italic>in vitro</italic> effects of IB‐367 alone and in combination with three standard antifungal drugs, fluconazole (FLU), itraconazole (ITRA) and terbinafine (TERB), against 20 clinical isolates of dermatophytes belonging to three species. Minimum inhibitory concentrations (MICs), minimal fungicidal concentrations (MFCs), synergy test, time‐kill curves, fungal biomass (FB) and hyphal damage using 2, 3‐bis‐(2‐methoxy‐4‐nitro‐5‐sulfenylamino carbonil)‐2H‐tetrazolium hydroxide assay (XTT) were performed to study the efficacy of IB‐367. In this study, we observed that TERB and ITRA had MICs lower values for all the strains compared to IB‐367 and FLU. Synergy was found in 35%, 30% and 25% of IB‐367/FLU, IB‐367/ITRA and IB‐367/TERB interactions respectively. IB‐367 exerted a fungicidal activity against <italic>Trichophyton mentagrophytes</italic>, <italic> T. rubrum</italic> and <italic>Microsporum canis</italic> at concentrations starting from 1x MIC. At a concentration of 5x MIC, IB‐367 showed the highest rates of hyphae damage for <italic>M. canis</italic> 53% and <italic>T. mentagrophytes</italic> 50%; against the same isolates it caused a reduction of 1 log of the total viable count cell hyphae damage. We propose IB‐367 as a promising candidate for the future design of antifungal drugs.</p> </abstract>