Identification of functionally important conserved trans‐membrane residues of bacterial PIB‐type ATPases. Issue 4 (14th January 2014)

• Record Type: Journal Article
• Authors: Zhitnitsky, Daniel; Lewinson, Oded
• Is Part Of: Molecular microbiology. Volume 91:Issue 4(2014)
• Page Start: 777
• Page End: 789
• Publication Date: 2014-01-14
• Subjects: Molecular microbiology -- Periodicals; 572.829
• DOI: 10.1111/mmi.12495
• Languages: English
• ISSNs: 0950-382X

Abstract

<abstract abstract-type="main"> <title>Summary</title> <p>Powered by ATP hydrolysis, P<sub>IB</sub>‐ATPases drive the energetically uphill transport of transition metals. These high affinity pumps are essential for heavy metal detoxification and delivery of metal cofactors to specific cellular compartments. Amino acid sequence alignment of the trans‐membrane (TM) helices of P<sub>IB</sub>‐ATPases reveals a high degree of conservation, with ∼60–70 fully conserved positions. Of these conserved positions, 6–7 were previously identified to be important for transport. However, the functional importance of the majority of the conserved TM residues remains unclear. To investigate the role of conserved TM residues of P<sub>IB</sub>‐ATPases we conducted an extensive mutagenesis study of a Zn<sup>2+</sup>/Cd<sup>2+</sup> P<sub>IB</sub>‐ATPase from <italic>R</italic><italic>hizobium radiobacter</italic> (rrZntA) and seven other P<sub>IB</sub>‐ATPases. Of the 38 conserved positions tested, 24 had small effects on metal tolerance. Fourteen mutations compromised <italic>in vivo</italic> metal tolerance and <italic>in vitro</italic> metal‐stimulated ATPase activity. Based on structural modelling, the functionally important residues line a constricted 'channel', tightly surrounded by the residues that were found to be inconsequential for function. We tentatively propose that the distribution of the mutable and immutable residues marks a possible trans‐membrane metal translocation pathway. In addition, by substituting six trans‐membrane amino acids of rrZntA we changed the <italic>in vivo</italic> metal specificity of this pump from Zn<sup>2+</sup>/Cd<sup>2+</sup> to Ag<sup>+</sup>.</p> </abstract>