MALDI‐TOF MS for identification of porcine Brachyspira species. (18th November 2013)

• Record Type: Journal Article
• Authors: Prohaska, S.; Pflüger, V.; Ziegler, D.; Scherrer, S.; Frei, D.; Lehmann, A.; Wittenbrink, M.M.; Huber, H.
• Is Part Of: Letters in applied microbiology. Volume 58:Number 3(2014:Mar.)
• Page Start: 292
• Page End: 298
• Publication Date: 2013-11-18
• Subjects: Microbiology -- Periodicals; 660.62
• DOI: 10.1111/lam.12189
• Languages: English
• ISSNs: 0266-8254

Abstract

<abstract abstract-type="main" id="lam12189-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="lam12189-sec-0101" sec-type="section"> <title>Abstract</title> <p>The aim of this study consisted in evaluating MALDI‐TOF MS as a tool for the identification of the genus <italic>Brachyspira</italic> (<italic>B</italic>.) and its relevant species for the pig industry. First, a database was created with 30 control strains, and superspectra for five different porcine <italic>Brachyspira</italic> species were calculated. In a second step, 67 field isolates were investigated using MALDI‐TOF MS, and results were compared to those obtained using <italic>nox</italic> gene‐based RFLP (reference method) and biochemical tests. Among the 67 field isolates, five different <italic>Brachyspira</italic> species were detected using <italic>nox</italic> gene‐based RFLP analysis. MALDI‐TOF MS analysis correctly assigned all isolates to the genus <italic>Brachyspira</italic> and identified all isolates from <italic>B. hyodysenteriae</italic> (29/29), <italic>B. pilosicoli</italic> (11/11), <italic>B. intermedia</italic> (4/4) and <italic>B. innocens</italic> (11/11). In terms of <italic>B. murdochii</italic>, MALDI‐TOF MS assigned one of 12 isolates ambiguously as <italic>B. innocens</italic>/<italic>B. murdochii</italic>. The results of this study indicate that MALDI‐TOF MS facilitates the diagnosis of swine dysentery and porcine intestinal spirochaetosis.</p> </sec> <sec id="lam12189-sec-0102" sec-type="section"> <title>Significance and Impact of the Study</title> <p>Current methods for the discrimination of pathogenic <italic>Brachyspira hyodysenteriae</italic> and <italic>Brachyspira pilosicoli</italic> from <italic>Brachyspira</italic> species with low pathogenic potential have proven to be laborious and time‐consuming and are therefore not suitable for routine diagnostics. This study describes the evaluation of MALDI‐TOF MS for the identification of different porcine <italic>Brachyspira</italic> species in routine diagnostic laboratories. The results suggest that MALDI‐TOF MS is an effective method for the identification of porcine <italic>Brachyspira</italic> spp. and accelerates diagnosis of swine dysentery and porcine intestinal spirochaetosis.</p> </sec> </abstract>