Oral administration of formalin killed Vibrio anguillarum cells improves growth and protection against challenge with Vibrio harveyi in banana shrimp. (14th November 2013)

• Record Type: Journal Article
• Authors: Patil, P.K.; Gopal, C.; Panigrahi, A.; Rajababu, D.; Pillai, S.M.
• Is Part Of: Letters in applied microbiology. Volume 58:Number 3(2014:Mar.)
• Page Start: 213
• Page End: 218
• Publication Date: 2013-11-14
• Subjects: Microbiology -- Periodicals; 660.62
• DOI: 10.1111/lam.12176
• Languages: English
• ISSNs: 0266-8254

Abstract

<abstract abstract-type="main" id="lam12176-abs-0001"> <title>Abstract</title> <sec id="lam12176-sec-0101" sec-type="section"> <p>Larval rearing in hatcheries and highly intensive grow‐out culture practices followed in shrimp production systems favour the growth of potential pathogenic bacterial loads. This study reports the efficacy of formalin‐killed vibrio bacterin on growth, survival and protection to challenge with virulent <italic>Vibrio harveyi</italic> and <italic>Vibrio anguillarum</italic> in juveniles of banana shrimp <italic>Fenneropenaeus merguiensis</italic>. Postlarvae 15 (0·24 ± 0·01 g) were administered orally in different concentrations of bacterial preparation (0, 10<sup>6</sup>, 10<sup>8</sup>, 10<sup>10</sup> and 10<sup>12 </sup>CFU kg<sup>−1</sup> feed) for a period of 6 weeks. Physicochemical and microbial quality of water in larval rearing tanks, and growth and survival of the postlarvae were monitored at regular intervals, and body composition was estimated at the end of the experiment. Shrimps were challenged with <italic>V. harveyi</italic> and <italic>V. anguillarum, </italic> and cumulative mortality was calculated. The group receiving 10<sup>8</sup> CFU kg<sup>−1</sup> feed showed highest average weight gain (162·66 ± 22·94 mg) and survival (90·33 ± 4·5%) and lowest cumulative mortality following the challenge with <italic>V. anguillarum</italic> (26%) and <italic>V. harveyi</italic> (36·67%). The results of the study suggest that formalized vibrio administered orally to <italic>F. merguiensis</italic> postlarvae could induce both homologous and heterologous protection against <italic>V. anguillarum</italic> and <italic>V. harveyi</italic>. 'Vaccination' of shrimp postlarvae at hatcheries would help in preventing the losses due to vibriosis and the most susceptible stages of shrimp development.</p> </sec> <sec id="lam12176-sec-0102" sec-type="section"> <title>Significance and Impact of the Study</title> <p>The study demonstrates the cross‐protection offered by the oral feeding of formalin‐killed <italic>Vibrio anguillarum</italic> against pathogenic <italic>V. harveyi</italic> challenge at the early developmental stages of banana shrimp, <italic>Fenneropenaeus merguiensis</italic>.</p> </sec> </abstract>