'Prevalence and antimicrobial susceptibility of Listeria monocytogenes and methicillin‐resistant Staphylococcus aureus strains from raw meat and meat products in Zaria, Nigeria. (14th November 2013)

• Record Type: Journal Article
• Authors: Ndahi, M.D.; Kwaga, J.K.P.; Bello, M.; Kabir, J.; Umoh, V.J.; Yakubu, S.E.; Nok, A.J.
• Is Part Of: Letters in applied microbiology. Volume 58:Number 3(2014:Mar.)
• Page Start: 262
• Page End: 269
• Publication Date: 2013-11-14
• Subjects: Microbiology -- Periodicals; 660.62
• DOI: 10.1111/lam.12183
• Languages: English
• ISSNs: 0266-8254

Abstract

<abstract abstract-type="main" id="lam12183-abs-0001"> <title>Abstract</title> <sec id="lam12183-sec-0101" sec-type="section"> <p>The bacterial genera <italic>Listeria</italic> and <italic>Staphylococcus</italic> have been frequently isolated from food products and are responsible for a number of animal and human diseases. The aim of the study was to simultaneously isolate and characterize <italic>L. monocytogenes</italic> and <italic>Staphylococcus</italic> species from 300 samples of raw meat and meat products, to determine the susceptibility of the organisms to commonly used antimicrobial agents and to determine the presence of haemolysin A (<italic>hyl)</italic> virulence gene in <italic>L. monocytogenes</italic> and staphylococcal cassette chromosome <italic>mec</italic>A (SCC<italic>mec</italic>) gene in the <italic>Staph. aureus</italic> isolates using PCR. Of the 85 <italic>Listeria</italic> isolates tested, 12 <italic>L. monocytogenes</italic> were identified and tested for their sensitivity to 14 antimicrobial agents. All the 12 isolates (100%) were resistant to nine antimicrobial agents, but however sensitive to gentamicin. Only one isolate was found to harbour the <italic>hylA</italic> gene. Twenty‐nine isolates were confirmed as <italic>Staph. aureus</italic> by the Microbact 12S identification system and were all presumptively identified as methicillin‐resistant <italic>Staph. aureus</italic> species using oxacillin‐resistant <italic>Staph. aureus</italic> basal medium (ORSAB). The 29 <italic>Staph. aureus</italic> isolates were tested for their sensitivity to 16 antimicrobial agents, and 11 were resistant to methicillin. None of the 11 <italic>Staph. aureus</italic> isolates harboured the methicillin resistance, <italic>mecA</italic> gene.</p> </sec> <sec id="lam12183-sec-0102" sec-type="section"> <title>Significance and Impact of the Study</title> <p> <italic>Listeria monocytogenes</italic> and <italic>Staphylococcus aureus</italic> are important agents of foodborne diseases. Occurrence of these infectious agents was established in meat and meat products in Zaria, Nigeria. Majority of isolates obtained from this study, displayed multidrug resistance to commonly used antimicrobial agents, including methicillin resistance among the <italic>Staph. aureus</italic> isolates. The potential virulence of <italic>L. monocytogenes</italic> found in ready‐to‐eat food was documented by the carriage of <italic>hly</italic> A gene by one of the isolates. A different mechanism of methicillin resistance or different homologue of <italic>mec</italic> A gene may be circulating among Nigerian isolates.</p> </sec> </abstract>