Plasma and Urine Neutrophil Gelatinase–Associated Lipocalin (NGAL) in Dogs with Acute Kidney Injury or Chronic Kidney Disease. (13th January 2014)

• Record Type: Journal Article
• Authors: Steinbach, S.; Weis, J.; Schweighauser, A.; Francey, T.; Neiger, R.
• Is Part Of: Journal of veterinary internal medicine. Volume 28:Number 2(2014:Mar./Apr.)
• Page Start: 264
• Page End: 269
• Publication Date: 2014-01-13
• Subjects: Veterinary medicine -- Periodicals; 636.0896
• DOI: 10.1111/jvim.12282
• Languages: English
• ISSNs: 0891-6640

Abstract

<abstract abstract-type="main" id="jvim12282-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="jvim12282-sec-0001" sec-type="section"> <title>Background</title> <p>Neutrophil gelatinase–associated lipocalin (NGAL) is a protein that is used in human medicine as a real‐time indicator of acute kidney injury (AKI).</p> </sec> <sec id="jvim12282-sec-0002" sec-type="section"> <title>Hypothesis</title> <p>Dogs with AKI have significantly higher plasma NGAL concentration and urine NGAL‐to‐creatinine ratio (UNCR) compared with healthy dogs and dogs with chronic kidney disease (CKD).</p> </sec> <sec id="jvim12282-sec-0003" sec-type="section"> <title>Animals</title> <p>18 healthy control dogs, 17 dogs with CKD, and 48 dogs with AKI.</p> </sec> <sec id="jvim12282-sec-0004" sec-type="section"> <title>Methods</title> <p>Over a period of 1 year, all dogs with renal azotemia were prospectively included. Urine and plasma samples were collected during the first 24 hours after presentation or after development of renal azotemia. Plasma and urine NGAL concentrations were measured with a commercially available canine NGAL Elisa Kit (Bioporto® Diagnostic) and UNCR was calculated. A single‐injection plasma inulin clearance was performed in the healthy dogs.</p> </sec> <sec id="jvim12282-sec-0005" sec-type="section"> <title>Results</title> <p>Median (range) NGAL plasma concentration in healthy dogs, dogs with CKD, and AKI were 10.7 ng/mL (2.5–21.2), 22.0 ng/mL (7.7–62.3), and 48.3 ng/mL (5.7–469.0), respectively. UNCR was 2 × 10<sup>−8</sup> (0–46), 1, 424 × 10<sup>−8</sup> (385–18, 347), and 2, 366 × 10<sup>−8</sup> (36–994, 669), respectively. Dogs with renal azotemia had significantly higher NGAL concentrations and UNCR than did healthy dogs (<italic>P</italic> < .0001 for both). Plasma NGAL concentration was significantly higher in dogs with AKI compared with dogs with CKD (<italic>P</italic> = .027).</p> </sec> <sec id="jvim12282-sec-0006" sec-type="section"> <title>Conclusions and Clinical Importance</title> <p>Plasma NGAL could be helpful to differentiate AKI from CKD in dogs with renal azotemia.</p> </sec> </abstract>