Coagulation Factor and Hemostatic Protein Content of Canine Plasma after Storage of Whole Blood at Ambient Temperature. (27th January 2014)

• Record Type: Journal Article
• Authors: Walton, J.E.; Hale, A.S.; Brooks, M.B.; Boag, A.K.; Barnett, W.; Dean, R.
• Is Part Of: Journal of veterinary internal medicine. Volume 28:Number 2(2014:Mar./Apr.)
• Page Start: 571
• Page End: 575
• Publication Date: 2014-01-27
• Subjects: Veterinary medicine -- Periodicals; 636.0896
• DOI: 10.1111/jvim.12277
• Languages: English
• ISSNs: 0891-6640

Abstract

<abstract abstract-type="main" id="jvim12277-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="jvim12277-sec-0001" sec-type="section"> <title>Background</title> <p>Standard practice in canine blood banking is to produce fresh frozen plasma (FFP) by separating and freezing plasma produced from blood within 8 hours of collection. Within canine blood donation programs, this can limit the number of units collected.</p> </sec> <sec id="jvim12277-sec-0002" sec-type="section"> <title>Hypothesis/Objectives</title> <p>The aim was to compare the coagulation factor and hemostatic protein content (CF&HPC) of plasma produced from blood stored at ambient temperature for 8, 12, and 24 hours. Another aim was to compare the CF&HPC between Greyhound types and other breeds.</p> </sec> <sec id="jvim12277-sec-0003" sec-type="section"> <title>Animals</title> <p>None.</p> </sec> <sec id="jvim12277-sec-0004" sec-type="section"> <title>Methods</title> <p>In vitro study. A convenience sample of 58 units of canine blood from a blood donor pool was processed to prepare and freeze plasma 8, 12, or 24 hours following collection.</p> </sec> <sec id="jvim12277-sec-0005" sec-type="section"> <title>Results</title> <p>Regardless of time of processing, the units contained therapeutic CF&HPC. Frozen plasma prepared after 24 hours had significantly higher factor VIII (<italic>P</italic> = .014) and factor X (<italic>P</italic> = .03) when compared with the frozen plasma prepared at 8 hours. Factor X (<italic>P </italic>< .01), fibrinogen (<italic>P</italic> < .01), and vWF (<italic>P</italic> = .04) were significantly lower in plasma collected from Greyhound types than in plasma collected from other breeds.</p> </sec> <sec id="jvim12277-sec-0006" sec-type="section"> <title>Conclusions and Clinical Importance</title> <p>Storing whole blood for up to 24 hours is a suitable method for producing FFP. Lower values for some coagulation factors and hemostatic proteins in plasma produced from Greyhound types would not preclude these dogs as FFP donors.</p> </sec> </abstract>