Feline blood genotyping versus phenotyping, and detection of non‐AB blood type incompatibilities in UK cats. (8th February 2014)

• Record Type: Journal Article
• Authors: Tasker, S.; Barker, E. N.; Day, M. J.; Helps, C. R.
• Is Part Of: Journal of small animal practice. Volume 55:Number 4(2014:Apr.)
• Page Start: 185
• Page End: 189
• Publication Date: 2014-02-08
• Subjects: Veterinary medicine -- Periodicals; Veterinary Medicine -- Periodicals; 636.089
• DOI: 10.1111/jsap.12180
• Languages: English
• ISSNs: 0022-4510

Abstract

<abstract abstract-type="main" id="jsap12180-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="jsap12180-sec-0001" sec-type="section"> <title>OBJECTIVES</title> <p id="jsap12180-para-0001"> <bold>The aim of this study was to determine the agreement between AB blood phenotyping and genotyping and determine whether non‐AB blood type incompatibilities exist in UK cats</bold>.</p> </sec> <sec id="jsap12180-sec-0002" sec-type="section"> <title>METHODS</title> <p id="jsap12180-para-0002"> <bold>Blood samples underwent phenotyping (A, B or AB) using microplate agglutination, and genotyping (<italic>AA</italic>, <italic>Ab</italic> or <italic>bb</italic>) using pyrosequencing of a fragment of the cytidine monophospho‐<italic>N</italic>‐acetylneuraminic acid hydroxylase gene. Non‐AB blood type incompatibilities were investigated by cross‐matching against reference blood of the same phenotype</bold>.</p> </sec> <sec id="jsap12180-sec-0003" sec-type="section"> <title>RESULTS</title> <p id="jsap12180-para-0003"> <bold>Of 112 cats tested, 86 (77%) were blood phenotype A, 19 (17%) type B and 7 (6%) type AB. Genotype and initial phenotype agreed in 96% (107 of 112) of cats, but 5 were discordant; these were all B phenotype with either <italic>AA</italic> (n=2) or <italic>Ab</italic> (n=3) genotype. Two of the five cats had repeat blood samples tested: one was reclassified as phenotype A; the other remained phenotype B. Two cats had incompatibilities on minor cross‐match, but these were attributed to phenotyping errors</bold>.</p> </sec> <sec id="jsap12180-sec-0004" sec-type="section"> <title>CLINICAL SIGNIFICANCE</title> <p id="jsap12180-para-0004"> <bold>Unknown mutation(s) associated with phenotype B, resulting in false <italic>AA</italic> or <italic>Ab</italic> genotyping, were evident in a small number of cases in this study. No conclusive evidence for non‐AB blood type incompatibilities was found</bold>.</p> </sec> </abstract>