Rapid Detection and Typing of Xanthomonas campestris pv. vesicatoria by Pyrosequencing. Issue 3 (11th September 2013)

• Record Type: Journal Article
• Authors: Gan, Qinhua; Lu, Yandu; Shao, Xiuling; Sun, Tao; Li, Yan; Liu, Caixia
• Is Part Of: Journal of phytopathology. Volume 162:Issue 3(2014:Mar.)
• Page Start: 190
• Page End: 194
• Publication Date: 2013-09-11
• Subjects: Plant diseases -- Periodicals; 632
• DOI: 10.1111/jph.12171
• Languages: English
• ISSNs: 0931-1785

Abstract

<abstract abstract-type="main" id="jph12171-abs-0001"> <title>Abstract</title> <p>Taking <italic>Xanthomonas campestris</italic> pv. <italic>vesicatoria</italic> (Doidge) Dye, a pathogen with a wide geographical distribution, as a representative, pyrosequencing is shown for the first time to provide characteristic information of plant pathogenic bacteria strain‐specific sequences. Pyrosequencing‐based plant pathogen detection and typing technology is demonstrated to be rapid, highly specific and more sensitive than conventional technologies. The specificity of such assays has been validated by conventional DNA sequencing and metabolic fingerprinting. It is a starting point for the application and development of pyrosequencing in plant inspection and quarantine which underlie agricultural communication.</p> </abstract>