AFLP and RAPD Characterization of Phaeoacremonium aleophilum Associated with Vitis vinifera Decline in Spain. Issue 4 (23rd September 2013)

• Record Type: Journal Article
• Authors: Martín, Laura; Sáenz de Miera, Luis E.; Martín, María Teresa
• Is Part Of: Journal of phytopathology. Volume 162:Issue 4(2014:Apr.)
• Page Start: 245
• Page End: 257
• Publication Date: 2013-09-23
• Subjects: Plant diseases -- Periodicals; 632
• DOI: 10.1111/jph.12180
• Languages: English
• ISSNs: 0931-1785

Abstract

<abstract abstract-type="main" id="jph12180-abs-0001"> <title>Abstract</title> <p>The genetic diversity among Spanish isolates of the fungus <italic>Phaeoacremonium aleophilum, </italic> one of the major causes of grapevine decline, was determined using random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) techniques. Using RAPD, a large genetic variation was observed among 36 <italic>Pm</italic>. <italic>aleophilum</italic> single‐spore cultures, with 76 (82.6%) polymorphic bands generated by 12 RAPD primers. A neighbour‐joining dendrogram showing the RAPD patterns of diversity revealed four groups of haplotypes. The Bayesian and principal components clustering analysis revealed three groups of haplotypes. When more than one isolate of <italic>Pm</italic>. <italic>aleophilum</italic> was obtained from a single vine, different haplotypes were found. Seventeen single‐spore isolates were used for AFLP analysis. Five primer combinations produced 358 scorable markers, of which 309 (86.3%) were polymorphic. The analysis based on genetic distance as well as clustering analysis confirmed three main groups largely in agreement with those returned by the RAPD results. The Mantel correlation between the RAPD and AFLP distance matrices ranged from <italic>R </italic>=<italic> </italic>0.5931 to <italic>R </italic>=<italic> </italic>0.6294. The high level of haplotype diversity among the RAPD and AFLP markers suggests that sexual reproduction and genetic recombination may occur between <italic>Pm</italic>. <italic>aleophilum</italic> haplotypes in Spain. The AFLP approach revealed a greater number of polymorphic markers. A relationship between the genetic profile of the infecting isolate of <italic>Pm</italic>. <italic>aleophilum</italic> and the age or decline symptoms of the grapevines may exist.</p> </abstract>