Triheptanoin partially restores levels of tricarboxylic acid cycle intermediates in the mouse pilocarpine model of epilepsy. (2nd December 2013)

• Record Type: Journal Article
• Authors: Hadera, Mussie G.; Smeland, Olav B.; McDonald, Tanya S.; Tan, Kah Ni; Sonnewald, Ursula; Borges, Karin
• Is Part Of: Journal of neurochemistry. Volume 129:Number 1(2014:Apr.)
• Page Start: 107
• Page End: 119
• Publication Date: 2013-12-02
• Subjects: Neurochemistry -- Periodicals; 616.8042
• DOI: 10.1111/jnc.12610
• Languages: English
• ISSNs: 0022-3042

Abstract

<abstract abstract-type="main" id="jnc12610-abs-0001"> <title>Abstract</title> <p>Triheptanoin, the triglyceride of heptanoate, is anticonvulsant in various epilepsy models. It is thought to improve energy metabolism in the epileptic brain by re‐filling the tricarboxylic acid (TCA) cycle with C4‐intermediates (anaplerosis). Here, we injected mice with [1, 2‐<sup>13</sup>C]glucose 3.5–4 weeks after pilocarpine‐induced status epilepticus (SE) fed either a control or triheptanoin diet. Amounts of metabolites and incorporations of <sup>13</sup>C were determined in extracts of cerebral cortices and hippocampal formation and enzyme activity and mRNA expression were quantified. The percentage enrichment with two <sup>13</sup>C atoms in malate, citrate, succinate, and GABA was reduced in hippocampal formation of control‐fed SE compared with control mice. Except for succinate, these reductions were not found in triheptanoin‐fed SE mice, indicating that triheptanoin prevented a decrease of TCA cycle capacity. Compared to those on control diet, triheptanoin‐fed SE mice showed few changes in most other metabolite levels and their <sup>13</sup>C labeling. Reduced pyruvate carboxylase mRNA and enzyme activity in forebrains and decreased [2, 3‐<sup>13</sup>C]aspartate amounts in cortex suggest a pyruvate carboxylation independent source of C‐4 TCA cycle intermediates. Most likely anaplerosis was kept unchanged by carboxylation of propionyl‐CoA derived from heptanoate. Further studies are proposed to fully understand triheptanoin's effects on neuroglial metabolism and interaction. <boxed-text content-type="graphic" id="jnc12610-blkfxd-0001" position="anchor" orientation="portrait"><graphic position="anchor" mimetype="image" xlink:href="ark:/27927/pgg4vnwvg7j" orientation="portrait" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /></boxed-text></p> <p>In the hippocampal formation (HF) of a mouse epilepsy model, formation of citrate, GABA, succinate, fumarate, and malate from <sup>13</sup>C‐labeled glucose is reduced. Triheptanoin, the triglyceride of heptanoate, inhibits pyruvate carboxylase activity (PC, green arrow), but restores some of these metabolite levels (red arrows). The refilling of the TCA cycle via carboxylation of propionyl‐CoA is likely to contribute to triheptanoin's anticonvulsant effects.</p> </abstract>