Comparative genetic analysis of Arabidopsis purple acid phosphatases AtPAP10, AtPAP12, and AtPAP26 provides new insights into their roles in plant adaptation to phosphate deprivation. Issue 3 (March 2014)

• Record Type: Journal Article
• Authors: Wang, Liangsheng; Lu, Shan; Zhang, Ye; Li, Zheng; Du, Xiaoqiu; Liu, Dong
• Is Part Of: Journal of integrative plant biology. Volume 56:Issue 3(2014)
• Page Start: 299
• Page End: 314
• Publication Date: 2014-03
• Subjects: Plants -- Periodicals; Plants -- China -- Periodicals; Electronic journals; 580.5
• DOI: 10.1111/jipb.12184
• Languages: English
• ISSNs: 1672-9072

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <sec id="jipb12184-sec-0001" sec-type="section"> <p>Induction and secretion of acid phosphatases (APases) is thought to be an adaptive mechanism that helps plants survive and grow under phosphate (Pi) deprivation. In <italic>Arabidopsis</italic>, there are 29 purple acid phosphatase (AtPAP) genes. To systematically investigate the roles of different AtPAPs, we first identified knockout or knock‐down T‐DNA lines for all 29 <italic>AtPAP</italic> genes. Using these <italic>atpap</italic> mutants combined with in‐gel and quantitative APase enzyme assays, we demonstrated that AtPAP12 and AtPAP26 are two major intracellular and secreted APases in <italic>Arabidopsis</italic> while AtPAP10 is mainly a secreted APase. On Pi‐deficient (P−) medium or P− medium supplemented with the organophosphates ADP and fructose‐6‐phosphate (Fru‐6‐P), growth of <italic>atpap10</italic> was significantly reduced whereas growth of <italic>atpap12</italic> was only moderately reduced, and growth of <italic>atpap26</italic> was nearly equal to that of the wild type (WT). Overexpression of the <italic>AtPAP12</italic> or <italic>AtPAP26</italic> gene, however, caused plants to grow better on P− or P− medium supplemented with ADP or Fru‐6‐P. Interestingly, Pi levels are essentially the same for the WT and overexpressing lines, although these two types of plants have significantly different growth phenotypes. These results suggest that the APases may have other roles besides enhancing internal Pi recycling or releasing Pi from external organophosphates for plant uptake.</p> </sec> </abstract>