Hypothermia induced by adenosine 5′‐monophosphate attenuates injury in an L‐arginine‐induced acute pancreatitis rat model. Issue 4 (April 2014)

• Record Type: Journal Article
• Authors: Wang, Yunlong; Guo, Weiting; Li, Yuan; Pan, Xinting; Lv, Wenshan; Cui, Lingling; Li, Changgui; Wang, Yangang; Yan, Shengli; Zhang, Jidong; Liu, Bin
• Is Part Of: Journal of gastroenterology and hepatology. Volume 29:Issue 4(2014:Apr.)
• Page Start: 742
• Page End: 748
• Publication Date: 2014-04
• Subjects: Gastroenterology -- Periodicals; Digestive organs -- Diseases -- Periodicals; Liver -- Diseases -- Periodicals; Gastroenterology -- Periodicals; Liver Diseases -- Periodicals; 616.33
• DOI: 10.1111/jgh.12448
• Languages: English
• ISSNs: 0815-9319

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <sec id="jgh12448-sec-0001" sec-type="section"> <title>Background and Aim</title> <p>This study sought to investigate the effects of hypothermia induced by adenosine 5′‐monophosphate (5′‐AMP) on L‐arginine (L‐Arg)‐induced acute pancreatitis in rats.</p> </sec> <sec id="jgh12448-sec-0002" sec-type="section"> <title>Methods</title> <p>The rats were divided into four groups: the control group, the acute pancreatitis group, the 5′‐AMP pretreatment group, and the 5′‐AMP posttreatment group. Rats in all groups, except for the control group, received two injections of 2.5 g/kg body weight (intraperitoneally) L‐Arg, with an interval of 1 h between the injections. Subsequently, the rats were observed to assess whether hypothermia induced by 5′‐AMP could effectively inhibit inflammation associated with L‐Arg‐induced acute pancreatitis in rats.</p> </sec> <sec id="jgh12448-sec-0003" sec-type="section"> <title>Results</title> <p>Hypothermia induced by 5′‐AMP produced protective effects in our acute pancreatitis model. These effects exhibited the following manifestations: (i) a significant reduction in rat mortality rates; (ii) a significant decrease in the occurrence of pancreatic edema; (iii) significant reductions in serum amylase (<italic>P</italic> < 0.001), interleukin‐6 (<italic>P</italic> < 0.001), interleukin‐1β (<italic>P</italic> < 0.001) and tumor necrosis factor‐α (<italic>P</italic> < 0.001); (iv) the significant inhibition of nuclear factor‐κB (NF‐κB) activation in rats that were pre‐ and posttreated with 5′‐AMP compared with rats that were only injected with L‐Arg; and (v) significant decreases in the occurrence of pancreatic interstitial edema, inflammatory cell infiltration, hemorrhage, and acinar cell necrosis.</p> </sec> <sec id="jgh12448-sec-0004" sec-type="section"> <title>Conclusions</title> <p>Hypothermia induced by 5′‐AMP could inhibit the acute inflammatory reaction and NF‐κB activation associated with acute pancreatitis.</p> </sec> </abstract>