In vitro effect of cortisol and urotensin I on arginine vasotocin and isotocin secretion from pituitary cells of gilthead sea bream Sparus aurata. Issue 2 (9th January 2014)

• Record Type: Journal Article
• Authors: Kalamarz‐Kubiak, H.; Meiri‐Ashkenazi, I.; Kleszczyńska, A.; Rosenfeld, H.
• Is Part Of: Journal of fish biology. Volume 84:Issue 2(2014)
• Page Start: 448
• Page End: 458
• Publication Date: 2014-01-09
• Subjects: Fishes -- Periodicals; Fishes -- Great Britain -- Periodicals; 597
• DOI: 10.1111/jfb.12297
• Languages: English
• ISSNs: 0022-1112

Abstract

<abstract abstract-type="main" id="jfb12297-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <p id="jfb12297-para-0001">This study aimed at determining whether <italic>in vitro</italic> secretion of two neuropeptides, arginine vasotocin (AVT) and isotocin (IT), from pituitary cells of gilthead sea bream <italic>Sparus aurata</italic> was affected by cortisol and urotensin (UI). Pituitary cells were exposed to 1·4 × 10⁻⁸, 1·4 × 10⁻⁷ and 0·4 × 10⁻⁶ M cortisol and 10⁻¹², 10⁻¹⁰ and 10⁻⁸ M UI for 6, 24 and 48 h, respectively. AVT and IT contents were determined in the culture media by high‐performance liquid chromatography (HPLC). An increase in AVT secretion and a decrease in IT secretion were observed at all cortisol doses. UI increased AVT secretion after 6 h of incubation at all doses. After 24 h, however, only the highest dose of UI still displayed an effect. IT secretion was not influenced by UI. It was thus demonstrated that cortisol does influence AVT and IT secretion from <italic>S. aurata</italic> pituitary cells, while UI regulates AVT secretion, as a component of hypothalamic–pituitary–interrenal (HPI) axis in this species.</p> </abstract>