Early detection of thrombin activity in neuroinflammatory disease. Issue 2 (24th February 2014)

• Record Type: Journal Article
• Authors: Davalos, Dimitrios; Baeten, Kim M.; Whitney, Michael A.; Mullins, Eric S.; Friedman, Beth; Olson, Emilia S.; Ryu, Jae Kyu; Smirnoff, Dimitri S.; Petersen, Mark A.; Bedard, Catherine; Degen, Jay L.; Tsien, Roger Y.; Akassoglou, Katerina
• Is Part Of: Annals of neurology. Volume 75:Issue 2(2014:Feb.)
• Page Start: 303
• Page End: 308
• Publication Date: 2014-02-24
• Subjects: Neurology -- Periodicals; Pediatric neurology -- Periodicals; Nervous system -- Surgery -- Periodicals; 616.8
• DOI: 10.1002/ana.24078
• Languages: English
• ISSNs: 0364-5134

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Although multiple sclerosis (MS) has been associated with the coagulation system, the temporal and spatial regulation of coagulation activity in neuroinflammatory lesions is unknown. Using a novel molecular probe, we characterized the activity pattern of thrombin, the central protease of the coagulation cascade, in experimental autoimmune encephalomyelitis. Thrombin activity preceded onset of neurological signs, increased at disease peak, and correlated with fibrin deposition, microglial activation, demyelination, axonal damage, and clinical severity. Mice with a genetic deficit in prothrombin confirmed the specificity of the thrombin probe. Thrombin activity might be exploited for developing sensitive probes for preclinical detection and monitoring of neuroinflammation and MS progression. Ann Neurol 2014;75:303–308</p> </abstract>