17β‐Estradiol Protects Human Eyelid‐Derived Adipose Stem Cells against Cytotoxicity and Increases Transplanted Cell Survival in Spinal Cord injury. Issue 2 (22nd December 2013)

• Record Type: Journal Article
• Authors: Zhou, Jing; Lu, Ping; Ren, Hao; Zheng, Zefeng; Ji, Junfeng; Liu, Hua; Jiang, Fangzhen; Ling, Shucai; Heng, Boon Chin; Hu, Xueqing; Ouyang, HongWei
• Is Part Of: Journal of cellular and molecular medicine. Volume 18:Issue 2(2014)
• Page Start: 326
• Page End: 343
• Publication Date: 2013-12-22
• Subjects: Cytology; Medicine; Molecular Biology; Cytologie -- Périodiques; Médecine -- Périodiques; Biologie moléculaire -- Périodiques; Cytology -- Periodicals; Medicine -- Periodicals; Molecular biology -- Periodicals; 611.01805
• DOI: 10.1111/jcmm.12191
• Languages: English
• ISSNs: 1582-1838

Abstract

<abstract abstract-type="main" id="jcmm12191-abs-0001"> <title>Abstract</title> <p>Stem cell transplantation represents a promising strategy for the repair of spinal cord injury (SCI). However, the low survival rate of the grafted cells is a major obstacle hindering clinical success because of ongoing secondary injury processes, which includes excitotoxicity, inflammation and oxidative stress. Previous studies have shown that 17b‐estradiol (E2) protects several cell types against cytotoxicity. Thus, we examined the effects of E2 on the viability of human eyelid adipose‐derived stem cells (hEASCs) <italic>in vitro</italic> with hydrogen peroxide (H₂O₂)‐induced cell model and <italic>in vivo</italic> within a rat SCI model. Our results showed that E2 protected hEASCs against H₂O₂‐induced cell death <italic>in vitro</italic>, and enhanced the survival of grafted hEASCs <italic>in vivo</italic> by reducing apoptosis. Additionally, E2 also enhanced the secretion of growth factors by hEASCs, thereby making the local microenvironment more conducive for tissue regeneration. Overall, E2 administration enhanced the therapeutic efficacy of hEASCs transplantation and facilitated motor function recovery after SCI. Hence, E2 administration may be an intervention of choice for enhancing survival of transplanted hEASCs after SCI.</p> </abstract>