Evaluating the performance of a rapid antigen test for the detection of influenza virus in clinical specimens from children in Cameroon. Issue 2 (24th November 2013)

• Record Type: Journal Article
• Authors: Kenmoe, Sebastien; Tchendjou, Patrice; Moyo Tetang, Suzie; Mossus, Tatiana; Njankouo Ripa, Mohammed; Guillet, Marlène; Kfutwah, Anfumbom; Njouom, Richard
• Is Part Of: Influenza and other respiratory viruses. Volume 8:Issue 2(2014:Mar.)
• Page Start: 131
• Page End: 134
• Publication Date: 2013-11-24
• Subjects: Influenza -- Periodicals; Respiratory infections -- Periodicals; Virus diseases -- Periodicals; Influenza, Human -- Periodicals; Respiratory Tract Diseases -- Periodicals; Virus Diseases -- Periodicals; Grippe -- Périodiques; Appareil respiratoire -- Infections -- Périodiques; Maladies à virus -- Périodiques; 616.203
• DOI: 10.1111/irv.12210
• Languages: English
• ISSNs: 1750-2640

Abstract

<abstract abstract-type="main" id="irv12210-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <p>The performance of SD Bioline rapid antigen test (RAT) was evaluated using real‐time reverse transcription polymerase chain reaction (rRT‐PCR) as gold standard. A total of 718 nasal swabs, including 102 rRT‐PCR positive and 616 rRT‐PCR negative swabs, were tested. RAT demonstrates a sensitivity of 29·4% with a specificity of 100%. The positivity rate of RAT was highly associated with lower cycle threshold (Ct) values (<italic>P </italic>&lt;<italic> </italic>0·0001). The excellent specificity of the RAT allowed for the rapid identification of influenza cases. However, negative results should be verified by rRT‐PCR test because of limitations observed in sensitivity.</p> </abstract>