Effects of bacterial products on the activity of odontoblast‐like cells and their formation of type 1 collagen. (31st July 2013)

• Record Type: Journal Article
• Authors: Fransson, H.; Petersson, K.; Davies, J. R.
• Is Part Of: International endontic journal. Volume 47:Number 4(2014:Apr.)
• Page Start: 397
• Page End: 404
• Publication Date: 2013-07-31
• Subjects: Endodontics -- Periodicals; 617.6342
• DOI: 10.1111/iej.12160
• Languages: English
• ISSNs: 0143-2885

Abstract

<abstract abstract-type="main" id="iej12160-abs-0001"> <title>Abstract</title> <sec id="iej12160-sec-0001" sec-type="section"> <title>Aim</title> <p>To study how products released from different bacteria in a deep carious lesion affect the metabolic activity of odontoblast‐like cells and their ability to produce the major organic component of dentine, collagen 1.</p> </sec> <sec id="iej12160-sec-0002" sec-type="section"> <title>Methodology</title> <p>MDPC‐23 cells were exposed to supernatants from biofilm cultures of strains isolated from the deepest part of a carious lesion as well as from a clinical isolate of <italic>Enterococcus faecalis</italic>. Lipoteichoic acid (LTA) and lipopolysaccharide (LPS) were used for comparison. Cell activity was assessed using an methyl‐thiazolyl‐diphenyl tetrazolium bromide (MTT) assay, and collagen 1 levels were determined by ELISA.</p> </sec> <sec id="iej12160-sec-0003" sec-type="section"> <title>Results</title> <p>The lesion microflora was dominated by <italic>Lactobacillus spp</italic>. Neither extracellular products from the isolates nor LPS affected the activity of the MDPC‐23 cells, whereas extracellular products from <italic>E. faecalis</italic> and LTA significantly reduced total cell activity (<italic>P</italic> < 0.01). <italic>Enterococcus faecalis</italic> had an inhibitory effect upon collagen 1 production by the cells, whereas no such effect or even a slight stimulatory effect was seen for the isolates from the deep carious lesion.</p> </sec> <sec id="iej12160-sec-0004" sec-type="section"> <title>Conclusions</title> <p>These studies indicate that culture supernatants from <italic>E. faecalis</italic> reduced the metabolic activity of odontoblast‐like cells as shown using the MTT assay. No effect was seen for supernatants from biofilms of bacteria recovered from a deep carious lesion. Different bacteria varied in their effects upon collagen 1 production suggesting that the nature of the bacterial species in a carious lesion may have a direct influence upon the ability of the odontoblasts to produce tertiary dentine.</p> </sec> </abstract>