Fully automated dual‐color dual‐hapten silver in situ hybridization staining for MYC amplification: a diagnostic tool for discriminating secondary angiosarcoma. (22nd January 2014)

• Record Type: Journal Article
• Authors: Ko, Jennifer S.; Billings, Steven D.; Lanigan, Christopher P.; Buehler, Darya; Fernandez, Anthony P.; Tubbs, Raymond R.
• Is Part Of: Journal of cutaneous pathology. Volume 41:Number 3(2014:Mar.)
• Page Start: 286
• Page End: 292
• Publication Date: 2014-01-22
• Subjects: Skin -- Diseases -- Periodicals; Dermatology -- Periodicals; 616
• DOI: 10.1111/cup.12278
• Languages: English
• ISSNs: 0303-6987

Abstract

<abstract abstract-type="main" id="cup12278-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="cup12278-sec-0001" sec-type="section"> <title>Background</title> <p id="cup12278-para-0001"> <italic>MYC</italic> amplification occurs in post‐radiation and chronic lymphedema‐associated secondary angiosarcoma and some primary angiosarcomas. In this study, we tested the ability of automated dual‐color dual‐hapten <italic>in situ</italic> hybridization (DISH) staining to discriminate secondary angiosarcoma from radiation‐associated atypical vascular lesions (AVL), and to correlate with fluorescence <italic>in situ</italic> hybridization (FISH) for <italic>MYC</italic> amplification.</p> </sec> <sec id="cup12278-sec-0002" sec-type="section"> <title>Methods</title> <p id="cup12278-para-0002">Cases of secondary angiosarcoma, including 11 biopsies and 3 excisions from 11 patients, and 5 AVL biopsies from 5 patients, were examined by FISH and DISH. DISH staining was performed using the <sc>Dual</sc><sc>Color</sc><sc>Open</sc><sc>Probe</sc> software on a Ventana Benchmark XT automated slide stainer. Metallic black silver (<italic>MYC</italic>) and reference CHR8 red signals were qualitatively and semi‐quantitatively enumerated for tumor nuclei. Small and large clusters of silver signals were recorded as 6 or 12 signals, respectively. <italic>MYC</italic> amplification was defined as <italic>MYC</italic>/CHR8 ratio >2.0.</p> </sec> <sec id="cup12278-sec-0003" sec-type="section"> <title>Results</title> <p id="cup12278-para-0003">Where tissue was available for both DISH and FISH, all secondary angiosarcoma cases showed <italic>MYC</italic> amplification (11/11 = 100%) by both DISH and FISH. All AVL were negative for <italic>MYC</italic> amplification by both techniques (0/5 = 0%).</p> </sec> <sec id="cup12278-sec-0004" sec-type="section"> <title>Conclusion</title> <p id="cup12278-para-0004">In the current cohort, use of DISH identified all <italic>MYC</italic> amplified cases, and distinguished secondary angiosarcoma from AVL. DISH staining may be useful in distinguishing secondary angiosarcoma from AVL in challenging cases.</p> </sec> </abstract>