CMTM3 inhibits cell migration and invasion and correlates with favorable prognosis in gastric cancer. Issue 1 (28th November 2013)

• Record Type: Journal Article
• Authors: Su, Yu; Lin, Yi; Zhang, Lianhai; Liu, Baocai; Yuan, Wanqiong; Mo, Xiaoning; Wang, Xiaohong; Li, Henan; Xing, Xiaofang; Cheng, Xiaojing; Dong, Bin; Hu, Ying; Du, Hong; Zhu, Yubing; Ding, Ning; Li, Jiyou; Liu, Weili; Ma, Yongzhen; Qiu, Xiaoyan; Ji, Jiafu; Han, Wenling
• Is Part Of: Cancer science. Volume 105:Issue 1(2014:Jan.)
• Page Start: 26
• Page End: 34
• Publication Date: 2013-11-28
• Subjects: Cancer -- Periodicals; Neoplasms -- Periodicals; Research -- Periodicals; Electronic journals; 616.994005
• DOI: 10.1111/cas.12304
• Languages: English
• ISSNs: 1347-9032

Abstract

<abstract abstract-type="main" id="cas12304-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <p>The CKLF‐like MARVEL transmembrane domain containing 3 (<italic>CMTM3</italic>) gene is a novel tumor suppressor with frequent epigenetic inactivation. In this study, we showed the role played by <italic>CMTM3</italic> in gastric cancer cells as a tumor suppressor gene, and examined the correlation between <italic>CMTM3</italic> expression and clinicopathological parameters using immunohistochemistry in gastric cancer patients with different pathological stages (<italic>n</italic> = 350). We found that <italic>CMTM3</italic> expression was reduced or silenced by epigenetic regulation in gastric cell lines, and dramatically downregulated in primary gastric cancer tissues. Restoration of <italic>CMTM3</italic> significantly affected migration and invasion of AGS and SGC‐7901 cells (<italic>P </italic>< 0.001). <italic>In vivo</italic> experiments showed that peritoneal disseminated metastases were significantly suppressed by <italic>CMTM3</italic> (<italic>P </italic>< 0.001). We further showed that the expression of MMP2 and the phosphorylation of Erk1/2 were decreased when <italic>CMTM3</italic> was restored. In addition, by immunohistochemical staining, we found that the expression of <italic>CMTM3</italic> was remarkably weaker in gastric cancer tissues than in normal mucosae (<italic>P</italic> = 0.008), and was significantly correlated with gender (<italic>P = </italic>0.033), tumor depth (<italic>P = </italic>0.049), stage (<italic>P = </italic>0.021), and histological grade (<italic>P = </italic>0.022). More importantly, <italic>CMTM3</italic> expression was associated with prognosis in gastric cancer patients (<italic>P = </italic>0.041), and was a significant independent prognostic indicator (hazard ratio = 0.704, 95% confidence interval, 0.498–0.994; <italic>P</italic> = 0.046). Our findings indicate that <italic>CMTM3</italic> regulates migration and invasion of gastric cancer cells. Moreover, <italic>CMTM3</italic> is a candidate marker for prognosis of gastric cancer in the clinic.</p> </abstract>