Copy number aberrations using multicolour fluorescence in situ hybridization (FISH) for prognostication in non‐muscle‐invasive bladder cancer (NIMBC). (26th July 2013)

• Record Type: Journal Article
• Authors: Matsuyama, Hideyasu; Ikemoto, Kenzo; Eguchi, Satoshi; Oga, Atsunori; Kawauchi, Shigeto; Yamamoto, Yoshiaki; Kawai, Yoshihisa; Matsumoto, Hiroaki; Hara, Takahiko; Nagao, Kazuhiro; Sakano, Shigeru; Sasaki, Kohsuke
• Is Part Of: BJU international. Volume 113:Number 4(2014:Apr.)
• Page Start: 662
• Page End: 667
• Publication Date: 2013-07-26
• Subjects: Genitourinary organs -- Diseases -- Periodicals; Genitourinary organs -- Surgery -- Periodicals; Urology -- Periodicals; 616.6
• DOI: 10.1111/bju.12232
• Languages: English
• ISSNs: 1464-4096

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="bju12232-sec-0001" sec-type="section"> <title>Objective</title> <p> <list id="bju12232-list-0001" list-type="bullet"> <list-item> <p>To investigate if detection of copy number aberrations of chromosomes 3, 7, 9p21, and 17 using multicolour fluorescence <italic>in situ</italic> hybridization (FISH) predicts patient outcome in non‐muscle‐invasive bladder cancer (NMIBC).</p> </list-item> </list> </p> </sec> <sec id="bju12232-sec-0002" sec-type="section"> <title>Patients and Methods</title> <p> <list id="bju12232-list-0002" list-type="bullet"> <list-item> <p>In all, 118 bladder wash samples were prospectively collected from patients who underwent transurethral resection of bladder tumour (median age 50.5 years, male/female: 91/27, tumour grade 1/2/3: 18/52/42, stage pTis/Ta/T1: 8/62/42) from 2007 to 2010.</p> </list-item> <list-item> <p>The 118 samples were analysed using the UroVysion® kit to detect the copy numbers of chromosomes 3, 7, 9p21, and 17.</p> </list-item> <list-item> <p>The variant fraction (VF; the sum of the non‐modal copy number fraction of each chromosome) was defined as abnormal when the percentage was ≥16%. The percentage deletion of 9p21 (fraction of null or one copy number of the 9p21 locus) was defined as abnormal when the percentage was ≥12%.</p> </list-item> <list-item> <p>Maffezzini risk criteria were also analysed in our cohorts.</p> </list-item> </list> </p> </sec> <sec id="bju12232-sec-0003" sec-type="section"> <title>Results</title> <p> <list id="bju12232-list-0003" list-type="bullet"> <list-item> <p>There was recurrence in 57 (48.3%) patients and disease progression in 12 (10.1%), with a median follow‐up of 35.7 months.</p> </list-item> <list-item> <p>Multivariate analysis showed that the percentage 9p21 loss (>12%) was an independent prognostic factor for recurrence (<italic>P</italic> < 0.001, odds ratio [OR] 3.24, 95% confidence interval [CI] 1.85–5.62).</p> </list-item> <list-item> <p>For disease progression, tumour grade, positive urine cytology, concurrent carcinoma <italic>in situ</italic>, and a mean VF of >16% were significant prognostic factors in univariate analysis. In multivariate analysis, a mean VF of >16% was a prognostic factor for disease progression (<italic>P</italic> = 0.048, OR 6.07, 95% CI 1.02–57.45).</p> </list-item> </list> </p> </sec> <sec id="bju12232-sec-0004" sec-type="section"> <title>Conclusions</title> <p> <list id="bju12232-list-0004" list-type="bullet"> <list-item> <p>Multicolour‐FISH analysis using a commercially available kit could be a powerful tool not only for diagnosis, but also for prognostication in patients with NMIBC.</p> </list-item> </list> </p> </sec> </abstract>