Assessment of the cutaneous immune response during Arthroderma benhamiae and A. vanbreuseghemii infection using an experimental mouse model. (March 2014)

• Record Type: Journal Article
• Authors: Cambier, L.; Weatherspoon, A.; Defaweux, V.; Bagut, E.T.; Heinen, M.P.; Antoine, N.; Mignon, B.
• Is Part Of: British journal of dermatology. Volume 170:Number 3(2014:Mar.)
• Page Start: 625
• Page End: 633
• Publication Date: 2014-03
• Subjects: Dermatology -- Periodicals; Skin -- Diseases -- Periodicals; 616.5
• DOI: 10.1111/bjd.12673
• Languages: English
• ISSNs: 0007-0963

Abstract

<abstract abstract-type="main" id="bjd12673-abs-0001"> <title>Summary</title> <sec id="bjd12673-sec-0001" sec-type="section"> <title>Background</title> <p>Dermatophytoses are common but poorly understood skin infections. Most <italic>in vivo</italic> studies have been performed using the guinea pig as the experimental animal model, which has several limitations.</p> </sec> <sec id="bjd12673-sec-0002" sec-type="section"> <title>Objectives</title> <p>To develop a mouse model of dermatophytosis suitable for multiple purposes, including the investigation of immunity against dermatophytes.</p> </sec> <sec id="bjd12673-sec-0003" sec-type="section"> <title>Materials and methods</title> <p>Two peculiar fungal species, <italic>Arthroderma benhamiae</italic> and <italic>A. vanbreuseghemii</italic>, isolated from tinea in humans having contact with rodents were used for epicutaneous inoculation. During the infection, clinical and histopathological follow‐up were performed. The recruitment of immune cells was evaluated by immunofluorescence staining and the levels of cytokine mRNA were quantified by quantitative reverse transcriptase‐polymerase chain reaction in the skin of infected mice.</p> </sec> <sec id="bjd12673-sec-0004" sec-type="section"> <title>Results</title> <p>The skin symptoms and microscopic lesions, including the colonization of keratinized epidermal and follicular structures by both dermatophytes, were highly similar to those observed in guinea pig infection models and in natural infections, mimicking acute superficial tinea in humans. The dermal inflammatory cellular infiltrate consisted of macrophages, dendritic cells and especially polymorphonuclear neutrophils, which are one of the histological 'clues' to the diagnosis of dermatophytosis. The <italic>in situ</italic> cytokine profile was characterized by the overexpression of transforming growth factor‐β, interleukin (IL)‐1β and IL‐6 mRNA during infection, suggesting a role of the T‐helper 17 pathway in the establishment of immunity.</p> </sec> <sec id="bjd12673-sec-0005" sec-type="section"> <title>Conclusions</title> <p>Our new reproducible and validated mouse model of dermatophytosis is a modern <italic>in vivo</italic> tool that allows a more in‐depth understanding of the pathogenesis of human dermatophyte infections.</p> </sec> </abstract>