Determination of bacteria constituting ruminal fibrolytic consortia developed on orchard grass hay stem. (21st November 2013)

• Record Type: Journal Article
• Authors: Shinkai, Takumi; Ueki, Takaaki; Koike, Satoshi; Kobayashi, Yasuo
• Is Part Of: Animal science journal. Volume 85:Number 3(2014:Mar.)
• Page Start: 254
• Page End: 261
• Publication Date: 2013-11-21
• Subjects: Animal culture -- Periodicals; Livestock -- Periodicals; 636
• DOI: 10.1111/asj.12145
• Languages: English
• ISSNs: 1344-3941

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>To determine the relationship between <italic>Fibrobacter succinogenes</italic> and other rumen bacteria, the bacterial community structure on fiber was analyzed by using two different materials. These were ruminally incubated orchard grass hay stems without and with preincubation with <italic>F. succinogenes</italic> (natural and artificial consortia, respectively). The natural consortium mainly consisted of <italic>Firmicutes</italic> (56.6%) and <italic>Bacteroidetes</italic> (33.1%), while the artificial consortium showed a significantly higher proportion of <italic>Firmicutes</italic> (85.5%) and a lower proportion of <italic>Bacteroidetes</italic> (4.6%). At species or genus level, <italic>Butyrivibrio fibrisolvens, </italic> the U2 group<italic>, Ruminococcus albus</italic> and <italic>Lachnospiraceae incertae sedis</italic> made up a higher proportion in the artificial consortium. The most dominant bacterial group was the <italic>Butyrivibrio‐Pseudobutyrivibrio‐Lachnospiraceae incertae sedis</italic> group, which accounted for 19.7% in the natural and 29.5% in the artificial consortium. Within the genus <italic>Butyrivibrio</italic>, the phylogenetic groups SA and VA2 and phylogeny‐undefined <italic>Butyribivrio</italic>, but not VA1, were detected at high frequency in the artificial consortium. These results suggest that ecological and possibly functional relationships exist in the rumen among <italic>F. succinogenes</italic>, a subset of <italic>B. fibrisolvens</italic>, the U2 group<italic>, R. albus</italic> and <italic>Lachnospiraceae incertae sedis</italic>.</p> </abstract>