Expression of Natural Cytotoxicity Receptors on Peritoneal Fluid Natural Killer Cell and Cytokine Production by Peritoneal Fluid Natural Killer Cell in Women with Endometriosis. (5th February 2014)

• Record Type: Journal Article
• Authors: Funamizu, Ayano; Fukui, Atsushi; Kamoi, Mai; Fuchinoue, Kohei; Yokota, Megumi; Fukuhara, Rie; Mizunuma, Hideki
• Is Part Of: American journal of reproductive immunology. Volume 71:Number 4(2014:Apr.)
• Page Start: 359
• Page End: 367
• Publication Date: 2014-02-05
• Subjects: Human reproduction -- Immunological aspects -- Periodicals; 616.69206
• DOI: 10.1111/aji.12206
• Languages: English
• ISSNs: 1046-7408

Abstract

<abstract abstract-type="main" id="aji12206-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="aji12206-sec-0001" sec-type="section"> <title>Problem</title> <p>To investigate the relationship between the expression of natural cytotoxicity receptors (NCRs) on peritoneal fluid (PF) natural killer (NK) (pfNK) cells and cytokine production by pfNK cells in women with endometriosis.</p> </sec> <sec id="aji12206-sec-0002" sec-type="section"> <title>Method of study</title> <p>Peritoneal fluid was collected from women with endometriosis undergoing laparoscopic surgery (<italic>n</italic> = 21) and controls without endometriosis (<italic>n</italic> = 28). The expression of NK cell surface antigens such as CD16 and NCRs (NKp46, NKp44 and NKp30) on pfNK cells, and cytokines production by pfNK cells [tumor necrosis factor (TNF)‐α, IFN‐γ, IL‐4, IL‐10, GM‐CSF and transforming growth factor (TGF)‐β<sub>1</sub>] were measured using multicolor flow cytometry.</p> </sec> <sec id="aji12206-sec-0003" sec-type="section"> <title>Results</title> <p>The percentages of CD56<sup>+</sup>/NKp46<sup>+</sup> cells and CD56<sup>dim</sup>/NKp46<sup>+</sup> cells in severe endometriosis group were significantly lower than that in controls. TNF‐α and IFN‐γ production by pfNK cells in severe endometriosis group was significantly higher than those in controls.</p> </sec> <sec id="aji12206-sec-0004" sec-type="section"> <title>Conclusion</title> <p>The differential expression of NKp46, TNF‐α, and IFN‐γ on pfNK cells in women with severe endometriosis may allow the proliferation and angiogenesis of endometriotic cells.</p> </sec> </abstract>