Time Dependence of Elimination of Different PEth Homologues in Alcoholics in Comparison with Social Drinkers. (29th October 2013)

• Record Type: Journal Article
• Authors: Gnann, Heike; Thierauf, Annette; Hagenbuch, Friedemann; Röhr, Bernhard; Weinmann, Wolfgang
• Is Part Of: Alcoholism. Volume 38:Number 2(2014:Feb.)
• Page Start: 322
• Page End: 326
• Publication Date: 2013-10-29
• Subjects: Alcoholism -- Periodicals; Alcoholism -- Periodicals; Alcoolisme; Electronic journals; Périodique électronique (Descripteur de forme); Ressource Internet (Descripteur de forme); 616.861005
• DOI: 10.1111/acer.12277
• Languages: English
• ISSNs: 0145-6008

Abstract

<abstract abstract-type="main" id="acer12277-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="acer12277-sec-0001" sec-type="section"> <title>Background</title> <p>Phosphatidylethanol (PEth) is a direct marker of alcohol consumption, which has been known for almost 30 years. Each PEth molecule carries 2 fatty acids, which differ in chain length and degree of unsaturation. It is formed by means of phospholipase D in the presence of ethanol. Usually, this marker was used by quantification of the PEth homologue 16:0/18:1. The intention of this work was to get more information about the distribution and the quantity of the different PEth homologues.</p> </sec> <sec id="acer12277-sec-0002" sec-type="section"> <title>Methods</title> <p>Blood samples from 12 alcohol‐dependent subjects were collected and analyzed during withdrawal therapy. For comparison, blood from 78 healthy social drinkers was also analyzed. PEth analysis was performed as follows: after liquid–liquid extraction, the homologues were separated on a Luna Phenyl Hexyl column, injected to an HPLC system (1100 system; Agilent) and identified by ESI‐MS/MS (QTrap 2000; AB Sciex) using multiple reaction monitoring.</p> </sec> <sec id="acer12277-sec-0003" sec-type="section"> <title>Results</title> <p>PEth 16:0/18:1 is the major homologue comparing the area ratios of PEth homologues in blood samples from alcoholics. Additional prevalent homologues were PEth 16:0/18:2, 18:0/18:2, and 18:0/18:1. The homologues occurring in blood samples from alcoholics as well as from social drinkers were mostly the same, but differences among their distribution pattern were observed.</p> </sec> <sec id="acer12277-sec-0004" sec-type="section"> <title>Conclusions</title> <p>In addition to the approach to quantitate the PEth homologue 16:0/18:1, this is a new and alternative proceeding for the differentiation between alcoholics and social drinkers using this alcohol consumption marker.</p> </sec> </abstract>