Interaction of Cryptosporidium parvum with mouse dendritic cells leads to their activation and parasite transportation to mesenteric lymph nodes. Issue 1 (10th September 2013)

• Record Type: Journal Article
• Authors: Perez‐Cordon, Gregorio; Yang, Guilin; Zhou, Boping; Nie, Weijia; Li, Shan; Shi, Lianfa; Tzipori, Saul; Feng, Hanping
• Is Part Of: Pathogens and disease. Volume 70:Issue 1(2014:Feb.)
• Page Start: 17
• Page End: 27
• Publication Date: 2013-09-10
• Subjects: Medical microbiology -- Periodicals; Pathogenic microorganisms -- Periodicals; Communicable diseases -- Microbiology -- Periodicals; Communicable diseases -- Pathogenesis -- Periodicals; Host-parasite relationships -- Periodicals; Systems biology -- Periodicals; 616.904105
• DOI: 10.1111/2049-632X.12078
• Languages: English
• ISSNs: 2049-632X

Abstract

<abstract abstract-type="main" id="fim12078-abs-0001"> <title>Abstract</title> <p>Dendritic cells (DCs) are the antigen‐presenting cells capable of activating naïve T cells. Although CD4+ T cells are crucial for <italic>Cryptosporidium parvum</italic> clearance, little is known about the role of DCs in the immune response to this parasite. In this study, the interaction between mouse DCs and <italic>C. parvum</italic> was investigated both <italic>in vitro</italic> and <italic>in vivo</italic>. For <italic>in vitro</italic> experiments, mouse bone marrow‐derived dendritic cells (BMDCs) derived from wild‐type C57B1/6 or MyD88−/− or C3H/HeJ mice and DC cell line DC2.4 were pulsed with <italic>C. parvum</italic>. Active invasion of parasites was demonstrated by parasite colocalization with host cell membranes and actin‐plaque formation at the site of attachment. DC activation induced by the parasite invasion was demonstrated by upregulation of costimulatory molecules CD40, CD80, and CD86, as well as inflammatory cytokines IL‐12, TNF‐α, and IL‐6. BMDCs derived from MyD88−/− and C3H/HeJ mice failed to produce IL‐12 in response to <italic>C. parvum</italic>, suggesting the importance of TLR‐dependent signaling pathway specially presence of a functional TLR4 pathway, for <italic>C. parvum</italic>‐induced cytokine production. <italic>In vivo</italic> experiments showed that both parasite antigens and live parasites were transported to mice mesenteric lymph nodes. All together, these data suggest that DCs play a key role in host immune responses to <italic>C. parvum</italic> and pathogenesis of the disease.</p> </abstract>