Dialysis Method Alters the Expression of MicroRNA‐33a and Its Target Genes ABCA1, ABCG1 in THP‐1 Macrophages. Issue 1 (February 2014)

• Record Type: Journal Article
• Authors: Wang, Jian‐Min; Zhou, Jia‐Jun; Zheng, Qian; Gan, Hua; Wang, Hang
• Is Part Of: Therapeutic apheresis and dialysis. Volume 18:Issue 1(2014)
• Page Start: 44
• Page End: 50
• Publication Date: 2014-02
• Subjects: Hemapheresis -- Periodicals; Dialysis -- Periodicals; Blood Component Removal -- Periodicals; Renal Dialysis -- Periodicals; Hémaphérèse -- Périodiques; Dialyse -- Périodiques; Sang -- Collecte et conservation -- Périodiques; 616
• DOI: 10.1111/1744-9987.12040
• Languages: English
• ISSNs: 1744-9979

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>Atherosclerosis and accompanying cardiovascular disease are the first causes of mortality in patients undergoing maintenance hemodialysis. Anti‐atherosclerotic effects of hemodiafiltration (HDF) have been reported. Our study aimed to investigate the effect of serum derived from a healthy group (<italic>n</italic> = 23), before and after hemodialysis (HD) therapy (<italic>n</italic> = 23), and before and after HDF therapy (<italic>n</italic> = 17) on the expression of microRNA‐33a and its target genes adenosine triphosphate‐binding cassette transporter A1, G1 (ABCA1, ABCG1) in THP‐1 macrophages. Meanwhile, blood lipids and high‐sensitivity C‐reactive protein (hs‐CRP) were measured in these groups. Our data showed that the expression of miRNA‐33a was lower (<italic>P</italic> < 0.05) and ABCA1 and ABCG1 were higher (<italic>P</italic> < 0.05) in the healthy group than pre‐HD and pre‐HDF. miR‐33a was significantly decreased (<italic>P</italic> < 0.05) but ABCA1, ABCG1 was significantly increased (<italic>P</italic> < 0.05) in post‐HDF compared with pre‐HDF, while these parameters in pre‐ and post‐ HD groups did not show any significant change (<italic>P</italic> > 0.05). High density lipoprotein cholesterol (HDL‐C) was higher and hs‐CRP was lower in the healthy group than pre‐HD and pre‐HDF groups. Moreover, a significant increase of HDL‐C (<italic>P</italic> < 0.05) and decrease (<italic>P</italic> < 0.05) of hs‐CRP was shown in post‐HDF compared with pre‐HDF, but HD appeared to have no significant change in these subjects. HDF therapy can downregulate miR‐33a expression, and then result in ABCA1, ABCG1 upregulation and an increase in circulating HDL‐C, leading to a possible anti‐atherosclerosis effect to some extent.</p> </abstract>