13C pulse‐chase labeling comparative assessment of the active methanogenic archaeal community composition in the transgenic and nontransgenic parental rice rhizospheres. Issue 3 (11th December 2013)

• Record Type: Journal Article
• Authors: Zhu, Weijing; Lu, Haohao; Hill, Jaclyn; Guo, Xiaohui; Wang, Hailong; Wu, Weixiang
• Is Part Of: FEMS microbiology ecology. Volume 87:Issue 3(2014)
• Page Start: 746
• Page End: 756
• Publication Date: 2013-12-11
• Subjects: Microbial ecology -- Periodicals; Microbiology -- Periodicals; 579.17
• DOI: 10.1111/1574-6941.12261
• Languages: English
• ISSNs: 0168-6496

Abstract

<abstract abstract-type="main" id="fem12261-abs-0001"> <title>Abstract</title> <p>More and more investigations indicate that genetic modification has no significant or persistent effects on microbial community composition in the rice rhizosphere. Very few studies, however, have focused on its impact on functional microorganisms. This study completed a <sup>13</sup>C‐CO<sub>2</sub> pulse‐chase labeling experiment comparing the potential effects of <italic>cry1Ab</italic> gene transformation on <sup>13</sup>C tissue distribution and rhizosphere methanogenic archaeal community composition with its parental rice variety (Ck) and a distant parental rice variety (Dp). Results showed that <sup>13</sup>C partitioning in aboveground biomass (mainly in stems) and roots of Dp was significantly lower than that of Ck. However, there were no significant differences in <sup>13</sup>C partitioning between the Bt transgenic rice line (Bt) and Ck. RNA‐stable isotope probing combined with clone library analyses inferred that the group <italic>Methanosaetaceae</italic> was the predominant methanogenic Archaea in all three rice rhizospheres. The active methanogenic archaeal community in the Bt rhizosphere was dominated by <italic>Methanosarcinaceae</italic>, <italic> Methanosaetaceae</italic>, and <italic>Methanomicrobiaceae</italic>, while there were only two main methanogenic clusters (<italic>Methanosaetaceae</italic> and <italic>Methanomicrobiaceae</italic>) in the Ck and Dp rhizospheres. These results indicate that the insertion of <italic>cry1Ab</italic> gene into the rice genome has the potential to result in the modification of methanogenic community composition in its rhizosphere.</p> </abstract>