Bacterial community variations in an alfalfa‐rice rotation system revealed by 16S rRNA gene 454‐pyrosequencing. Issue 3 (3rd December 2013)

• Record Type: Journal Article
• Authors: Lopes, Ana R.; Manaia, Célia M.; Nunes, Olga C.
• Is Part Of: FEMS microbiology ecology. Volume 87:Issue 3(2014)
• Page Start: 650
• Page End: 663
• Publication Date: 2013-12-03
• Subjects: Microbial ecology -- Periodicals; Microbiology -- Periodicals; 579.17
• DOI: 10.1111/1574-6941.12253
• Languages: English
• ISSNs: 0168-6496

Abstract

<abstract abstract-type="main" id="fem12253-abs-0001"> <title>Abstract</title> <p>Crop rotation is a practice harmonized with the sustainable rice production. Nevertheless, the implications of this empirical practice are not well characterized, mainly in relation to the bacterial community composition and structure. In this study, the bacterial communities of two adjacent paddy fields in the 3rd and 4th year of the crop rotation cycle and of a nonseeded subplot were characterized before rice seeding and after harvesting, using 454‐pyrosequencing of the 16S rRNA gene. Although the phyla <italic>Acidobacteria</italic>, <italic> Proteobacteria</italic>, <italic> Chloroflexi</italic>, <italic> Actinobacteria</italic> and <italic>Bacteroidetes</italic> predominated in all the samples, there were variations in relative abundance of these groups. Samples from the 3rd and 4th years of the crop rotation differed on the higher abundance of groups of presumable aerobic bacteria and of presumable anaerobic and acidobacterial groups, respectively. Members of the phylum <italic>Nitrospira</italic> were more abundant after rice harvest than in the previously sampled period. Rice cropping was positively correlated with the abundance of members of the orders <italic>Acidobacteriales</italic> and '<italic>Solibacterales</italic>' and negatively with lineages such as <italic>Chloroflexi</italic> 'Ellin6529'. Studies like this contribute to understand variations occurring in the microbial communities in soils under sustainable rice production, based on real‐world data.</p> </abstract>