Utilization of matrix‐assisted laser desorption and ionization time‐of‐flight mass spectrometry for identification of infantile seborrheic dermatitis‐causing Malassezia and incidence of culture‐based cutaneous Malassezia microbiota of 1‐month‐old infants. Issue 2 (3rd January 2014)

• Record Type: Journal Article
• Authors: Yamamoto, Mikachi; Umeda, Yoshiko; Yo, Ayaka; Yamaura, Mariko; Makimura, Koichi
• Is Part Of: Journal of dermatology. Volume 41:Issue 2(2014)
• Page Start: 117
• Page End: 123
• Publication Date: 2014-01-03
• Subjects: Dermatology -- Periodicals; Dermatology -- Japan -- Periodicals; Skin -- Diseases -- Periodicals; 616.5005
• DOI: 10.1111/1346-8138.12364
• Languages: English
• ISSNs: 0385-2407

Abstract

<abstract abstract-type="main" id="jde12364-abs-0001"> <title>Abstract</title> <p>Matrix‐assisted laser desorption and ionization time‐of‐flight mass spectrometry (MALDI‐TOF‐MS) has been utilized for identification of various microorganisms. <italic>Malassezia</italic> species, including <italic>Malassezia restricta</italic>, which is associated with seborrheic dermatitis, has been difficult to identify by traditional means. This study was performed to develop a system for identification of <italic>Malassezia</italic> species with MALDI‐TOF‐MS and to investigate the incidence and variety of cutaneous <italic>Malassezia</italic> microbiota of 1‐month‐old infants using this technique. A <italic>Malassezia</italic> species‐specific MALDI‐TOF‐MS database was developed from eight standard strains, and the availability of this system was assessed using 54 clinical strains isolated from the skin of 1‐month‐old infants. Clinical isolates were cultured initially on CHROMagar <italic>Malassezia</italic> growth medium, and the 28S ribosomal DNA (D1/D2) sequence was analyzed for confirmatory identification. Using this database, we detected and analyzed <italic>Malassezia</italic> species in 68% and 44% of infants with and without infantile seborrheic dermatitis, respectively. The results of MALDI‐TOF‐MS analysis were consistent with those of rDNA sequencing identification (100% accuracy rate). To our knowledge, this is the first report of a MALDI‐TOF‐MS database for major skin pathogenic <italic>Malassezia</italic> species. This system is an easy, rapid and reliable method for identification of <italic>Malassezia</italic>.</p> </abstract>