Immobilization of Actively Thromboresistant Assemblies on Sterile Blood‐Contacting Surfaces. Issue 1 (21st June 2013) - Qu, Zheng
Abstract Rapid one‐step modification of thrombomodulin with alkylamine derivatives such as azide, biotin, and PEG is achieved using an evolved sortase (eSrtA) mutant. The feasibility of a point‐of‐care scheme is demonstrated herein to site‐specifically immobilize azido‐thrombomodulin on sterilized commercial ePTFE vascular grafts, which exhibit superior thromboresistance compared with commercial heparin‐coated grafts in a primate model of acute graft thrombosis.