Nanovesicle‐Based Bioelectronic Nose for the Diagnosis of Lung Cancer from Human Blood. Issue 3 (18th July 2013)

• Record Type: Journal Article
• Authors: Lim, Jong Hyun; Park, Juhun; Oh, Eun Hae; Ko, Hwi Jin; Hong, Seunghun; Park, Tai Hyun
• Is Part Of: Advanced healthcare materials. Volume 3:Issue 3(2014:Mar.)
• Page Start: 360
• Page End: 366
• Publication Date: 2013-07-18
• Subjects: Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/adhm.201300174
• Languages: English
• ISSNs: 2192-2640

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p>A human nose‐mimetic diagnosis system that can distinguish the odor of a lung cancer biomarker, heptanal, from human blood is presented. Selective recognition of the biomarker is mimicked in the human olfactory system. A specific olfactory receptor recognizing the chemical biomarker is first selected through screening a library of human olfactory receptors (hORs). The selected hOR is expressed on the membrane of human embryonic kidney (HEK)‐293 cells. Nanovesicles containing the hOR on the membrane are produced from these cells, and are then used for the functionalization of single‐walled carbon nanotubes. This strategy allows the development of a sensitive and selective nanovesicle‐based bioelectronic nose (NvBN). The NvBN is able to selectively detect heptanal at a concentration as low as 1 × 10⁻¹⁴<sc>m</sc>, a sufficient level to distinguish the blood of a lung cancer patient from the blood of a healthy person. In actual experiments, NvBN could detect an extremely small increase in the amount of heptanal from human blood plasma without any pretreatment processes. This result offers a rapid and easy method to analyze chemical biomarkers from human blood in real‐time and to diagnose lung cancer.</p> </abstract>