RGD‐Conjugated Nanoscale Coordination Polymers for Targeted T1‐ and T2‐weighted Magnetic Resonance Imaging of Tumors in Vivo. (25th October 2013)

• Record Type: Journal Article
• Authors: Yang, Hong; Qin, Changyuan; Yu, Chao; Lu, Yang; Zhang, Hongwei; Xue, Fengfeng; Wu, Dongmei; Zhou, Zhiguo; Yang, Shiping
• Is Part Of: Advanced functional materials. Volume 24:Number 12(2014)
• Page Start: 1738
• Page End: 1747
• Publication Date: 2013-10-25
• Subjects: Materials -- Periodicals; Chemical vapor deposition -- Periodicals; 620.11
• DOI: 10.1002/adfm.201302433
• Languages: English
• ISSNs: 1616-301X

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Development of multifunctional nanoscale coordination polymers (NCPs) allowing for <italic>T</italic><sub>1</sub>‐ and <italic>T</italic><sub>2</sub>‐weighted targeted magnetic resonance (MR) imaging of tumors could significantly improve the diagnosis accuracy. In this study, nanoscale coordination polymers (NCPs) with a diameter of ≈80 nm are obtained with 1, 1′‐dicarboxyl ferrocene (Fc) as building blocks and magnetic gadolinium(III) ions as metallic nodes using a nanoprecipitation method, then further aminated through silanization. The amine‐functionalized Fc‐Gd@SiO<sub>2</sub> NCPs enable the covalent conjugation of a fluorescent rhodamine dye (RBITC) and an arginine‐glycine‐aspartic acid (RGD) peptide as a targeting ligand onto their surface. The formed water‐dispersible Fc‐Gd@SiO<sub>2</sub>(RBITC)–RGD NCPs exhibit a low cytotoxicity, as confirmed by MTT assay. They have a longitudinal relaxivity (<italic>r</italic><sub>1</sub>) of 5.1 mM<sup>−1</sup> s<sup>−1</sup> and transversal relaxivity (<italic>r</italic><sub>2</sub>) of 21.7 mM<sup>−1</sup> s<sup>−1</sup>, suggesting their possible use as both <italic>T</italic><sub>1</sub>‐positive and <italic>T</italic><sub>2</sub>‐negative contrast agents. In vivo MR imaging experiments show that the signal of tumor over‐expressing high affinity <italic>α<sub>v</sub>β<sub>3</sub></italic> integrin from <italic>T</italic><sub>1</sub>‐weighted MR imaging is positively enhanced 47±5%, and negatively decreased 33±5% from <italic>T</italic><sub>2</sub>‐weighted MR imaging after intravenous injection of Fc‐Gd@SiO<sub>2</sub>(RBITC)–RGD NCPs.</p> </abstract>