Probing Physical Properties of a DNA‐Protein Complex Using Nanofluidic Channels. Issue 5 (2nd January 2014)

• Record Type: Journal Article
• Authors: Frykholm, Karolin; Alizadehheidari, Mohammadreza; Fritzsche, Joachim; Wigenius, Jens; Modesti, Mauro; Persson, Fredrik; Westerlund, Fredrik
• Is Part Of: Small. Volume 10:Issue 5(2014:Mar.)
• Page Start: 884
• Page End: 887
• Publication Date: 2014-01-02
• Subjects: Nanotechnology -- Periodicals; Nanoparticles -- Periodicals; Microtechnology -- Periodicals; 620.5
• DOI: 10.1002/smll.201302028
• Languages: English
• ISSNs: 1613-6810

Abstract

<abstract abstract-type="graphical" xml:lang="en" id="smll201302028-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <bold>A method to investigate physical properties of a DNA‐protein complex in solution</bold> is demonstrated. By using tapered nanochannels and lipid passivation the persistence length of a RecA filament formed on double‐stranded DNA is determined to 1.15 μm, in agreement with the literature, without attaching protein or DNA to any handles or surfaces. <boxed-text content-type="graphic" position="anchor" orientation="portrait"><graphic position="anchor" mimetype="image" xlink:href="ark:/27927/pgg4sz3ktvf" orientation="portrait" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /></boxed-text></p> </abstract>