A Facile Method for Preparation of Tailored Scaffolds for DNA‐Origami. Issue 1 (17th July 2013)

• Record Type: Journal Article
• Authors: Erkelenz, Michael; Bauer, Dennis M.; Meyer, Rebecca; Gatsogiannis, Christos; Raunser, Stefan; Saccà, Barbara; Niemeyer, Christof M.
• Is Part Of: Small. Volume 10:Issue 1(2014:Jan.)
• Page Start: 73
• Page End: 77
• Publication Date: 2013-07-17
• Subjects: Nanotechnology -- Periodicals; Nanoparticles -- Periodicals; Microtechnology -- Periodicals; 620.5
• DOI: 10.1002/smll.201300701
• Languages: English
• ISSNs: 1613-6810

Abstract

<abstract abstract-type="graphical" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <bold>A convenient PCR cloning strategy allows one to prepare hundreds of picomoles of circular single‐stranded DNA</bold> molecules, which are suitable as scaffolds for the assembly of DNA origami structures. The method is based on a combination of site‐directed mutagenesis and site‐ and ligation‐independent cloning protocols, with simultaneous insertion of a nicking endonuclease restriction site on a double‐stranded plasmid of desired length and sequence. <boxed-text content-type="graphic" position="anchor" orientation="portrait"><graphic position="anchor" mimetype="image" xlink:href="ark:/27927/pgg4sqn5gw9" orientation="portrait" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /></boxed-text></p> </abstract>