Leucine supplementation modulates fuel substrates utilization and glucose metabolism in previously obese mice. (5th September 2013)

• Record Type: Journal Article
• Authors: Binder, Elke; Bermúdez‐Silva, Francisco Javier; Elie, Melissa; Leste‐Lasserre, Thierry; Belluomo, Ilaria; Clark, Samantha; Duchampt, Adeline; Mithieux, Gilles; Cota, Daniela
• Is Part Of: Obesity. Volume 22:Number 3(2014:Mar.)
• Page Start: 713
• Page End: 720
• Publication Date: 2013-09-05
• Subjects: Obesity -- Periodicals; 616.398005
• DOI: 10.1002/oby.20578
• Languages: English
• ISSNs: 1930-7381

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="oby20578-sec-0001" sec-type="section"> <title>Objective</title> <p>High‐protein diets favor weight loss and its maintenance. Whether these effects might be recapitulated by certain amino acids is unknown. Therefore, the impact of leucine supplementation on energy balance and associated metabolic changes in diet‐induced obese (DIO) mice during and after weight loss was investigated.</p> </sec> <sec id="oby20578-sec-0002" sec-type="section"> <title>Methods</title> <p>DIO C57BL/6J mice were fed a normocaloric diet to induce weight loss while receiving or not the amino acid leucine in drinking water. Body weight, food intake, body composition, energy expenditure, glucose tolerance, insulin, and leptin sensitivity were evaluated. Q‐PCR analysis was performed on muscle, brown and white adipose tissues.</p> </sec> <sec id="oby20578-sec-0003" sec-type="section"> <title>Results</title> <p>DIO mice decreased body weight and fat mass in response to chow, but supplementation with leucine did not affect these parameters. During weight maintenance, mice supplemented with leucine had improved glucose tolerance, increased leptin sensitivity, and lower respiratory quotient. The latter was associated with changes in the expression of several genes modulating fatty acid metabolism and mitochondrial activity in the epididymal white and the brown adipose tissues, but not muscle.</p> </sec> <sec id="oby20578-sec-0004" sec-type="section"> <title>Conclusions</title> <p>Leucine supplementation might represent an adjuvant beneficial nutritional therapy during weight loss and maintenance, because it improves lipid and glucose metabolism and restores leptin sensitivity in previously obese animals.</p> </sec> </abstract>