In vivo imaging of islet transplantation using PLGA nanoparticles containing iron oxide and indocyanine green. Issue 3 (2nd May 2013)

• Record Type: Journal Article
• Authors: Hwang, Jung Hwan; Noh, Young‐Woock; Choi, Jung‐Hyun; Noh, Jung‐Ran; Kim, Yong‐Hoon; Gang, Gil‐Tae; Kim, Kyoung‐Shim; Park, Hye Sun; Lim, Yong Taik; Moon, Hyeyoung; Hong, Kwan Soo; Lee, Hee Gu; Chung, Bong Hyun; Lee, Chul‐Ho
• Is Part Of: Magnetic resonance in medicine. Volume 71:Issue 3(2014:Mar.)
• Page Start: 1054
• Page End: 1063
• Publication Date: 2013-05-02
• Subjects: Nuclear magnetic resonance -- Periodicals; Electron paramagnetic resonance -- Periodicals; 616.07548
• DOI: 10.1002/mrm.24768
• Languages: English
• ISSNs: 0740-3194

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="mrm24768-sec-0001" sec-type="section"> <title>Purpose</title> <p>We determined whether poly(lactic‐<italic>co</italic>‐glycolic acid) nanoparticles would be a useful reagent for the successful monitoring of isolated islets by magnetic resonance imaging and optical imaging systems, without clinically relevant toxicity in vitro or in vivo.</p> </sec> <sec id="mrm24768-sec-0002" sec-type="section"> <title>Methods</title> <p>We used iron oxide for MR imaging and a cyanide dye approved by the Food and Drug Administration (indocyanine green) for optical imaging and estimated the in vivo detection of transplanted pancreatic islets.</p> </sec> <sec id="mrm24768-sec-0003" sec-type="section"> <title>Results</title> <p>The poly(lactic‐<italic>co</italic>‐glycolic acid) nanoparticles were associated with the islets in vitro and were successfully detected by 4.7 T (MR) and optical imaging, without other toxic effects. When labeled islets were transplanted under the mouse kidney capsule, in vivo <italic>T</italic><sub>2</sub><italic>/</italic><alternatives><inline-graphic mimetype="image" xlink:href="ark:/27927/pgg4sw31djq" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /><mml:math display="inline" altimg="urn:x-wiley:07403194:media:mrm24768:mrm24768-math-0001" overflow="scroll" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mrow><mml:msubsup><mml:mi>T</mml:mi><mml:mn>2</mml:mn><mml:mo>*</mml:mo></mml:msubsup></mml:mrow></mml:math></alternatives>‐weighted scans with 4.7 T MR detected as few as 300 labeled islets by 4 weeks. Optical in vivo imaging revealed indocyanine green fluorescence by 2 and 4 days after transplantation of islets containing 250 and 500 µg/mL poly(lactic‐<italic>co</italic>‐glycolic acid) nanoparticles, respectively. These results were further supported by the immunohistochemical results for insulin and iron in the recipient mouse kidney and pancreas.</p> </sec> <sec id="mrm24768-sec-0004" sec-type="section"> <title>Conclusions</title> <p>Taken together, these data indicate that poly(lactic‐<italic>co</italic>‐glycolic acid) nanoparticles may be used to label transplanted islets and may be imaged with in vivo MR and optical imaging systems. <bold>Magn Reson Med 71:1054–1063, 2014. © 2013 Wiley Periodicals, Inc.</bold></p> </sec> </abstract>