Gastric cancer does not affect the expression of atrophy‐related genes in human skeletal muscle. Issue 4 (10th February 2014)

• Record Type: Journal Article
• Authors: D'Orlando, Cristina; Marzetti, Emanuele; François, Stephanie; Lorenzi, Maria; Conti, Valentina; di, Enrico; Rosa, Fausto; Brunelli, Silvia; Doglietto, Giovan Battista; Pacelli, Fabio; Bossola, Maurizio
• Is Part Of: Muscle & nerve. Volume 49:Issue 4(2014:Apr.)
• Page Start: 528
• Page End: 533
• Publication Date: 2014-02-10
• Subjects: Neuromuscular diseases -- Periodicals; Muscles -- Periodicals; Nerves -- Periodicals; 616.74
• DOI: 10.1002/mus.23945
• Languages: English
• ISSNs: 0148-639X

Abstract

<abstract abstract-type="main"> <title>ABSTRACT</title> <p> <italic>Introduction</italic>: We evaluated the gene expression levels of <italic>atrogin‐1</italic>, <italic>MuRF1</italic>, <italic>myostatin</italic>, <italic>follistatin</italic>, <italic>activin A</italic>, and <italic>inhibin alpha</italic> in skeletal muscle samples of patients with gastric cancer and controls. <italic>Methods</italic>: We studied 38 cancer patients and 12 controls who underwent surgery for gastric adenocarcinoma and benign abdominal diseases, respectively. A biopsy specimen was obtained from the rectus abdominis muscle from all participants. The relative gene expression of <italic>atrogin‐1</italic>, <italic>MuRF1</italic>, <italic>myostatin</italic>, <italic>follistatin</italic>, <italic>activin A</italic>, and <italic>inhibin alpha</italic> was determined by quantitative real‐time polymerase chain reaction analysis. <italic>Results</italic>: <italic>Atrogin‐1</italic> and <italic>MuRF1</italic> mRNA expression was similar between cancer patients and controls and was unaffected by the disease stage or the severity of body weight loss. Transcript levels of <italic>myostatin</italic> and <italic>follistatin</italic> did not differ between cases and controls and were similar across disease stages and categories of weight loss. Finally, no differences were detected in <italic>activin A</italic> and <italic>inhibin alpha</italic> gene expression between cancer patients and controls. <italic>Conclusions</italic>: In skeletal muscle, the gene expression of <italic>atrogin‐1</italic>, <italic>MuRF1</italic>, <italic>myostatin</italic>, <italic>follistatin</italic>, <italic>activin A</italic>, and <italic>inhibin alpha</italic> is not affected by the presence of cancer. The expression of atrophy‐related genes is unaffected by the disease stage and the degree of weight loss. Muscle Nerve 49:528–533, 2014</p> </abstract>