Improved wound healing of postischemic cutaneous flaps with the use of bone marrow‐derived stem cells. (19th July 2013)

• Record Type: Journal Article
• Authors: Hu, Melissa; Ludlow, David; Alexander, J. Steven; McLarty, Jerry; Lian, Timothy
• Is Part Of: Laryngoscope. Volume 124:Number 3(2014:Mar.)
• Page Start: 642
• Page End: 648
• Publication Date: 2013-07-19
• Subjects: Otolaryngology -- Periodicals; 617.51005
• DOI: 10.1002/lary.24293
• Languages: English
• ISSNs: 0023-852X

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="lary24293-sec-0001" sec-type="section"> <title>Objectives/Hypothesis</title> <p>To determine if the intravascular delivery of mesenchymal stem cells improves wound healing and blood perfusion to postischemic cutaneous flap tissues.</p> </sec> <sec id="lary24293-sec-0002" sec-type="section"> <title>Study Design</title> <p>Randomized controlled study.</p> </sec> <sec id="lary24293-sec-0003" sec-type="section"> <title>Methods</title> <p>A murine model of a cutaneous flap was created based on the inferior epigastric vessels. Mice (n = 14) underwent 3.5 hours of ischemia followed by reperfusion. Bone marrow stromal cells (BMSCs) 1 × 10<sup>6</sup> were injected intravenously. Wound healing was then assessed measuring percent flap necrosis, flap perfusion, and tensile strength of the flap after a period of 14 days. Localization of BMSCs was determined with radiolabeled and fluorescent labeled BMSCs.</p> </sec> <sec id="lary24293-sec-0004" sec-type="section"> <title>Results</title> <p>Postischemic cutaneous flap tissues treated with BMSCs demonstrated significantly less necrosis than control flaps (<italic>P</italic> <0.01). Beginning on postoperative day 5, BMSC‐treated flaps demonstrated greater blood perfusion than untreated flaps (<italic>P</italic> <0.01). Tensile strength of BMSC‐treated cutaneous flaps was significantly higher (<italic>P</italic> <0.01), with a mean strength of 283.4 ± 28.4 N/m than control flaps with a mean of 122.4 ± 23.5 N/m. Radiolabeled BMSCs localized to postischemic flaps compared to untreated tissues (<italic>P</italic> = 0.001). Fluorescent microscopy revealed incorporation of BMSCs into endothelial and epithelial tissues of postischemic flaps.</p> </sec> <sec id="lary24293-sec-0005" sec-type="section"> <title>Conclusions</title> <p>This study demonstrates that the intravascular delivery of BMSCs increases wound healing and promotes flap survival following ischemia‐reperfusion injury of cutaneous tissue flaps. <italic>Laryngoscope</italic>, 124:642–648, 2014</p> </sec> </abstract>