MALDI‐TOF mass spectrometry, an efficient technique for in situ detection and characterization of actinomycins. (17th February 2014)

• Record Type: Journal Article
• Authors: Vater, Joachim; Crnovčić, Ivana; Semsary, Siamak; Keller, Ullrich
• Is Part Of: Journal of mass spectrometry. Volume 49:Number 3(2014:Mar.)
• Page Start: 210
• Page End: 222
• Publication Date: 2014-02-17
• Subjects: Mass spectrometry -- Periodicals; 543.65
• DOI: 10.1002/jms.3329
• Languages: English
• ISSNs: 1076-5174

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>An extensive study of actinomycins was performed using matrix‐assisted laser desorption/ionization mass spectrometry (MALDI‐TOF MS). Actinomycins represent a well‐known family of peptidolactone chromopeptides with potent cytostatic and antibiotic properties. Using five well‐characterized streptomycete strains, we introduced MALDI‐TOF MS as an efficient technique for rapid <italic>in situ</italic> detection of actinomycins in surface extracts of cells picked from agar plates. By this procedure, actinomycin complexes can be investigated with high sensitivity and accuracy in a minimum of time. These studies were complemented by mass spectrometric investigation of actinomycins obtained from culture filtrate extracts and purified by high‐performance liquid chromatography to detect yet unknown actinomycin species. By feeding experiments, C‐demethyl‐actinomycins from <italic>Streptomyces chrysomallus</italic> and <italic>Streptomyces parvulus</italic> as well as <italic>hemi</italic>‐actinomycins from <italic>Streptomyces antibioticus</italic> lacking one of the two pentapeptide lactone rings were isolated and characterized as novel variants for structure–activity relationship studies. Structural characterization of the investigated actinomycins was performed by post source decay MALDI‐TOF MS. The specific features of the fragmentation patterns of the protonated and cationized forms of selected actinomycins were investigated in detail. Copyright © 2014 John Wiley & Sons, Ltd.</p> </abstract>