Development of an integrated optical analyzer for characterization of growth dynamics of bacterial colonies. Issue 11 (18th April 2013)

• Record Type: Journal Article
• Authors: Kim, Huisung; Bai, Nan; Bhunia, Arun K.; King, Galen B.; Hirleman, E. Daniel; Bae, Euiwon
• Is Part Of: Journal of biophotonics. Volume 6:Issue 11/12(2013:Dec.)
• Page Start: 929
• Page End: 937
• Publication Date: 2013-04-18
• Subjects: Photonics -- Periodicals; Optical materials -- Periodicals; Optics -- Periodicals; Medical instruments and apparatus -- Periodicals; 621.3605
• DOI: 10.1002/jbio.201200224
• Languages: English
• ISSNs: 1864-063X

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>In order to understand the biophysics behind collective behavior of a bacterial colony, a confocal displacement meter was used to measure the profiles of the bacterial colonies, together with a custom built optical density circuits. The system delivered essential information related to the quantitative growth dynamics (height, diameter, aspect ratio, optical density) of the bacterial colony. For example, the aspect ratio of <italic>S. aureus</italic> was approximately two times higher than that of <italic>E. coli</italic> O157 : H7, while the OD of <italic>S. aureus</italic> was approximately 1/3 higher than that of <italic>E. coli</italic> O157 : H7. (© 2013 WILEY‐VCH Verlag GmbH &amp; Co. KGaA, Weinheim)</p> </abstract>