Global DNA methylation status in laryngeal cancer. Issue 3 (1st June 2013)

• Record Type: Journal Article
• Authors: Stembalska, Agnieszka; Leszczyński, Przemysław; Gil, Justyna; Ramsey, David; Pitala, Grzegorz; Maciejczyk, Adam; Frączek, Marcin
• Is Part Of: Head & neck. Volume 36:Issue 3(2014:Mar.)
• Page Start: 419
• Page End: 424
• Publication Date: 2013-06-01
• Subjects: Head -- Diseases -- Periodicals; Neck -- Diseases -- Periodicals; Head -- Periodicals; Neck -- Periodicals; Face -- Periodicals; 617.51059
• DOI: 10.1002/hed.23315
• Languages: English
• ISSNs: 1043-3074

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="hed23315-sec-0001" sec-type="section"> <title>Background</title> <p>The purpose of our study was to evaluate if DNA methylation level in leukocytes may be used as a surrogate marker of genome methylation status in laryngeal cancer tissues.</p> </sec> <sec id="hed23315-sec-0002" sec-type="section"> <title>Methods</title> <p>We evaluated global DNA methylation using an ultra performance liquid chromatography (UPLC)‐based method to assess the total content of 5‐methylcytosine (5 mC).</p> </sec> <sec id="hed23315-sec-0003" sec-type="section"> <title>Results</title> <p>The study was performed on DNA isolated from cancer tissues, adjacent normal tissues, and peripheral blood leukocytes in a group of 72 patients with laryngeal cancer. DNA hypomethylation was found in tumor tissue (56%) and normal tissue (49%). There was a significant correlation between the levels of 5 mC in these 2 types of tissue. There was no significant DNA hypomethylation in blood. A negative correlation between tumor grade and blood levels of 5 mC was found.</p> </sec> <sec id="hed23315-sec-0004" sec-type="section"> <title>Conclusion</title> <p>The level of leukocyte DNA methylation measured using total 5 mC content cannot be used as a surrogate marker for genome methylation status in laryngeal cancer tissues. © 2013 Wiley Periodicals, Inc. <italic>Head Neck</italic><bold>36</bold>: 419–424, 2014</p> </sec> </abstract>