Dose‐dependent effect of mitomycin C on human vocal fold fibroblasts12. Issue 3 (14th June 2013)

• Record Type: Journal Article
• Authors: Li, Nicole Y. K.; Chen, Fei; Dikkers, Frederik G.; Thibeault, Susan L.
• Is Part Of: Head & neck. Volume 36:Issue 3(2014:Mar.)
• Page Start: 401
• Page End: 410
• Publication Date: 2013-06-14
• Subjects: Head -- Diseases -- Periodicals; Neck -- Diseases -- Periodicals; Head -- Periodicals; Neck -- Periodicals; Face -- Periodicals; 617.51059
• DOI: 10.1002/hed.23310
• Languages: English
• ISSNs: 1043-3074

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="hed23310-sec-0001" sec-type="section"> <title>Background</title> <p>The purpose of this study was to evaluate in vitro cytotoxicity and antifibrotic effects of mitomycin C on normal and scarred human vocal fold fibroblasts.</p> </sec> <sec id="hed23310-sec-0002" sec-type="section"> <title>Methods</title> <p>Fibroblasts were subjected to mitomycin C treatment at 0.2, 0.5, or 1 mg/mL, or serum control. Cytotoxicity, immunocytochemistry, and Western blot for collagen I/III were performed at days 0, 1, 3, and 5.</p> </sec> <sec id="hed23310-sec-0003" sec-type="section"> <title>Results</title> <p>Significant decreases in live cells were measured for mitomycin C‐treated cells on days 3 and 5 for all doses. Extracellular staining of collagen I/III was observed in mitomycin C‐treated cells across all doses and times. Extracellular staining suggests apoptosis with necrosis, compromising the integrity of cell membranes and release of cytosolic proteins into the extracellular environment. Western blot indicates inhibition of collagen at all doses except 0.2 mg/mL at day 1.</p> </sec> <sec id="hed23310-sec-0004" sec-type="section"> <title>Conclusion</title> <p>A total of 0.2 mg/mL mitomycin C may provide initial and transient stimulation of collagen for necessary repair to damaged tissue without the long‐term risk of fibrosis. <italic>Head Neck</italic><bold>36</bold>: 401–410, 2014</p> </sec> </abstract>