Proteomics of a fuzzy organelle: interphase chromatin. (17th February 2014)

• Record Type: Journal Article
• Authors: Kustatscher, Georg; Hégarat, Nadia; Wills, Karen L H; Furlan, Cristina; Bukowski‐Wills, Jimi‐Carlo; Hochegger, Helfrid; Rappsilber, Juri
• Is Part Of: EMBO journal. Volume 33:Number 6(2014)
• Page Start: 648
• Page End: 664
• Publication Date: 2014-02-17
• Subjects: Molecular biology -- Periodicals; 572.805
• DOI: 10.1002/embj.201387614
• Languages: English
• ISSNs: 0261-4189

Abstract

<abstract abstract-type="synopsis" xml:lang="en" id="embj201387614-abs-0002"> <title>Synopsis</title> <p> <boxed-text content-type="graphic" id="embj201387614-blkfxd-0003" position="anchor" orientation="portrait"> <graphic position="anchor" mimetype="image" xlink:href="ark:/27927/pgg4st0ck6f" orientation="portrait" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /> </boxed-text> </p> <p>A probabilistic definition of interphase chromatin captures its dynamic and regulated composition with a new proteomics‐based quantitative protein ontology approach. This resource is subsequently applied for the identification of novel factors whose interphase chromatin association depends on cyclin‐dependent kinase (CDK).</p> <p> <list id="embj201387614-list-0001" list-type="bullet"> <list-item> <p>Static protein lists in organellar proteomics are replaced by degrees of chromatin involvement.</p> </list-item> <list-item> <p>Chromatin components are defined by biological co‐behavior instead of biochemical co‐purification.</p> </list-item> <list-item> <p>Interphase chromatin probabilities (ICPs) for 7635 proteins focus datasets on likely chromatin factors.</p> </list-item> <list-item> <p>Chromatin enrichment for proteomics (ChEP) defines a simple but efficient procedure to enrich chromatin for proteomic analysis.</p> </list-item> <list-item> <p>Proteomic analysis of CDK‐dependent chromatin composition reveals novel cell cycle‐regulated chromatin factors.</p> </list-item> </list> </p> </abstract>