Polo‐like kinase‐1 triggers histone phosphorylation by Haspin in mitosis. (13th January 2014)

• Record Type: Journal Article
• Authors: Zhou, Linli; Tian, Xiaoying; Zhu, Cailei; Wang, Fangwei; Higgins, Jonathan MG
• Is Part Of: EMBO reports. Volume 15:Number 3(2014:Mar.)
• Page Start: 273
• Page End: 281
• Publication Date: 2014-01-13
• Subjects: Molecular biology -- Periodicals; Molecular Biology -- Periodicals; Molecular biology; Periodicals; 572.8
• DOI: 10.1002/embr.201338080
• Languages: English
• ISSNs: 1469-221X

Abstract

<abstract abstract-type="main" id="embr201338080-abs-0001"> <title>Abstract</title> <p>Histone modifications coordinate the chromatin localization of key regulatory factors in mitosis. For example, mitotic phosphorylation of Histone H3 threonine‐3 (H3T3ph) by Haspin creates a binding site for the chromosomal passenger complex (CPC). However, how these histone modifications are spatiotemporally controlled during the cell cycle is unclear. Here we show that Plk1 binds to Haspin in a Cdk1‐phosphorylation‐dependent manner. Reducing Plk1 activity decreases the phosphorylation of Haspin and inhibits H3T3ph, particularly in prophase, suggesting that Plk1 is required for initial activation of Haspin in early mitosis. These studies demonstrate that Plk1 can positively regulate CPC recruitment in mitosis.</p> </abstract>