Biological Production of 2‐Butanone in Escherichia coli. Issue 1 (5th November 2013)

• Record Type: Journal Article
• Authors: Yoneda, Hisanari; Tantillo, Dean J.; Atsumi, Shota
• Is Part Of: ChemSusChem. Volume 7:Issue 1(2014:Jan.)
• Page Start: 92
• Page End: 95
• Publication Date: 2013-11-05
• Subjects: Green chemistry -- Periodicals; Sustainable engineering -- Periodicals; Chemistry -- Periodicals; Chemical engineering -- Periodicals; 660
• DOI: 10.1002/cssc.201300853
• Languages: English
• ISSNs: 1864-5631

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>An <italic>Escherichia coli</italic> (<italic>E. coli</italic>) strain was engineered to synthesize 2‐butanone from glucose by extending the 2, 3‐butanediol synthesis reaction sequence catalyzed by exogenous enzymes. To convert 2, 3‐butanediol to 2‐butanone, B12‐dependent glycerol dehydratase from <italic>Klebsiella pneumoniae</italic> was introduced into <italic>E. coli</italic>. It has been proposed that the enzyme has a weak activity toward 2, 3‐butanediol. The activity in <italic>E. coli</italic> is confirmed in this study. Furthermore, co‐expressing coenzyme B12 reactivators increased the 2‐butanone titer. This demonstration of 2‐butanone production by extending the 2, 3‐butanediol biosynthetic pathway provides the possibility to produce this valuable chemical renewably.</p> </abstract>