Multi‐Color Imaging of Fluorescent Nanodiamonds in Living HeLa Cells Using Direct Electron‐Beam Excitation. Issue 4 (8th January 2014)

• Record Type: Journal Article
• Authors: Nawa, Yasunori; Inami, Wataru; Lin, Sheng; Kawata, Yoshimasa; Terakawa, Susumu; Fang, Chia‐Yi; Chang, Huan‐Cheng
• Is Part Of: Chemphyschem. Volume 15:Issue 4(2014)
• Page Start: 721
• Page End: 726
• Publication Date: 2014-01-08
• Subjects: Chemistry, Physical and theoretical -- Periodicals; 541.05
• DOI: 10.1002/cphc.201300802
• Languages: English
• ISSNs: 1439-4235

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>Multi‐color, high spatial resolution imaging of fluorescent nanodiamonds (FNDs) in living HeLa cells has been performed with a direct electron‐beam excitation‐assisted fluorescence (D‐EXA) microscope. In this technique, fluorescent materials are directly excited with a focused electron beam and the resulting cathodoluminescence (CL) is detected with nanoscale resolution. Green‐ and red‐light‐emitting FNDs were employed for two‐color imaging, which were observed simultaneously in the cells with high spatial resolution. This technique could be applied generally for multi‐color immunostaining to reveal various cell functions.</p> </abstract>