Epidithiodiketopiperazine Biosynthesis: A Four‐Enzyme Cascade Converts Glutathione Conjugates into Transannular Disulfide Bridges1. Issue 42 (26th August 2013)

• Record Type: Journal Article
• Authors: Scharf, Daniel H.; Chankhamjon, Pranatchareeya; Scherlach, Kirstin; Heinekamp, Thorsten; Willing, Karsten; Brakhage, Axel A.; Hertweck, Christian
• Is Part Of: Angewandte Chemie international edition. Volume 52:Issue 42(2013)
• Page Start: 11092
• Page End: 11095
• Publication Date: 2013-08-26
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201305059
• Languages: English
• ISSNs: 1433-7851

Abstract

<abstract abstract-type="graphical" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <bold>Enzyme quartet</bold>: Isolation of the first sulfur‐bearing intermediate of the gliotoxin pathway in <italic>Aspergillus fumigatus</italic> and successful in vitro conversion of the bisglutathione adduct into an intact epidithiodiketopiperazine by a four‐enzyme cascade (including glutamyltransferase GliK and dipeptidase GliJ) revealed an outstanding adaptation of a primary metabolic pathway into natural product biosynthesis that is widespread in fungi.<boxed-text content-type="graphic" position="anchor" orientation="portrait"><graphic position="anchor" mimetype="image" xlink:href="ark:/27927/pgg3kg2jk0p" orientation="portrait" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /></boxed-text></p> </abstract>