Rigid DNA Beams for High‐Resolution Single‐Molecule Mechanics1. Issue 30 (21st June 2013)

• Record Type: Journal Article
• Authors: Pfitzner, Emanuel; Wachauf, Christian; Kilchherr, Fabian; Pelz, Benjamin; Shih, William M.; Rief, Matthias; Dietz, Hendrik
• Is Part Of: Angewandte Chemie international edition. Volume 52:Issue 30(2013)
• Page Start: 7766
• Page End: 7771
• Publication Date: 2013-06-21
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201302727
• Languages: English
• ISSNs: 1433-7851

Abstract

<abstract abstract-type="graphical" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <bold>Bridging the gap</bold>: Rigid DNA linkers (blue, see picture) between microspheres (green) for high‐resolution single‐molecule mechanical experiments were constructed using DNA origami. The resulting DNA helical bundles greatly reduce the noise generated in studies of conformation changes using optical tweezers and were applied to study small DNA secondary structures.<boxed-text content-type="graphic" position="anchor" orientation="portrait"><graphic position="anchor" mimetype="image" xlink:href="ark:/27927/pgg270w4h2s" orientation="portrait" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /></boxed-text></p> </abstract>