Improved Super‐Resolution Microscopy with Oxazine Fluorophores in Heavy Water1. Issue 34 (4th July 2013)

• Record Type: Journal Article
• Authors: Lee, Steven F.; Vérolet, Quentin; Fürstenberg, Alexandre
• Is Part Of: Angewandte Chemie international edition. Volume 52:Issue 34(2013)
• Page Start: 8948
• Page End: 8951
• Publication Date: 2013-07-04
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201302341
• Languages: English
• ISSNs: 1433-7851

Abstract

<abstract abstract-type="graphical" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <bold>Brighter dyes in heavy water</bold>: A simple and cost‐effective method increases the brightness of a whole class of commonly used red‐emitting fluorophores, including ATTO655, ATTO680, and ATTO700. Replacing water (H₂O) by heavy water (D₂O) in the imaging buffer doubles the fluorescence quantum yield of these dyes and significantly improves the localization precision in super‐resolution imaging.<boxed-text content-type="graphic" position="anchor" orientation="portrait"><graphic position="anchor" mimetype="image" xlink:href="ark:/27927/pgg36jz8hqp" orientation="portrait" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /></boxed-text></p> </abstract>